SAB4200543
Anti-Calbindin-D-28K antibody, Mouse monoclonal
clone CB-955, purified from hybridoma cell culture
别名:
Monoclonal Anti-(28kD), Monoclonal Anti-D-28K, Monoclonal Anti-D-dependent calcium-binding protein, Monoclonal Anti-RTVL-H protein, Monoclonal Anti-avian-type, Monoclonal Anti-calbindin 1, Monoclonal Anti-vitamin
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关于此项目
NACRES:
NA.44
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
CB-955, monoclonal
Application:
IHC, WB
Citations:
17
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
CB-955, monoclonal
form
buffered aqueous solution
mol wt
antigen ~28 kDa
species reactivity
mouse, human, rat
enhanced validation
independent
Learn more about Antibody Enhanced Validation
concentration
~1.0 mg/mL
technique(s)
immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin embedded rat cerebellum., western blot: 2-4 μg/mL using MDBK extract.
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CALB1(793)
General description
Anti-Calbindin-D-28K antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma CB-955 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified bovine kidney calbindin-D-28K.
Calbindin-D-28K (CALB1) is encoded by the gene mapped to human chromosome 8q21.3-q22.1. CALB1 belongs to the EF hand superfamily of Ca2+ binding proteins (CBPs). The encoded protein contains EF hand motif involved in the specific and reversible binding of Ca2+. It is highly expressed in specific regions of central nervous system such as cerebellar Purkinje cells and hippocampal granule cells.
Immunogen
purified bovine kidney calbindin-D-28K. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor.
Application
- Anti-Calbindin-D-28K antibody, Mouse monoclonal has been used in:
- western blotting
- enzyme-linked immunosorbent assay (ELISA){31
- immunohistochemistry
- immunohistochemical procedure
- indirect immunofluorescence
Biochem/physiol Actions
Calbindin D-28k might play a vital role as an intracellular Ca2+ buffer. It has an ability to protect nerve cells from calcium-mediated neurotoxicity. The encoded protein functions as a potent neuroanatomical marker which can be used to selectively visualize certain neurons and pathways in the central nervous system and peripheral nervous system. Mutations in the gene has been observed in patients with Parkinson′s disease (PD).
Calbindin-D (28K) functions as a mobile or partly immobilized Ca2+ -buffer with medium kinetics and affinity buffer function. Second, it functions in buffered Ca2+- diffusion-transport function. Finally, it interacts with target proteins in its apo-and Ca2+-loaded form-sensor- like function. Over-expression of this protein stimulates neurite outgrowth in dopaminergic neuronal cells.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Ai Takeshita et al.
Scientific reports, 8(1), 6917-6917 (2018-05-04)
Fibroblast growth factor 23 (FGF23) plays critical roles in phosphate handling and vitamin D metabolism in the kidney. However, the effector cells of FGF23 in the kidney remain unclear. αKlotho, a putative enzyme possessing β-glucuronidase activity and also a permissive
A comparative study of three interneuron types in the rat spinal cord
Chen S, et al.
PLoS ONE, 11(9), e0162969-e0162969 (2016)
Calbindin 1, fibroblast growth factor 20, and ?-synuclein in sporadic Parkinson?s disease
Mizuta I
Human Genetics, 124, 89-94 (2008)
The human calbindin D28k (CALB1) and calretinin (CALB2) genes are located at 8q21.3----q22.1 and 16q22----q23, respectively, suggesting a common duplication with the carbonic anhydrase isozyme loci.
Parmentier M
Cytogenetics and Cell Genetics, 57, 41-43 (1991)
Binding Kinetics of Calbindin-D28k Determined by Flash Photolysis of Caged Ca2+
Nagerl UV
Biophysical Journal, 79, 3009-3018 (2000)
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