SAB4200606
Anti-EB3 antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
别名:
Anti-EBF3, Anti-EBF3-S, Anti-MAPRE3, Anti-RP/EB family, Anti-RP3, Anti-member 3, Anti-microtubule-associated protein
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunoblotting
immunoprecipitation (IP)
immunoprecipitation (IP)
Species reactivity:
mouse, human, rat
Citations:
7
Technique(s):
immunoblotting: 1-2 μg/mL using lysates of rat brain (S1 fraction)
immunoprecipitation (IP): 5-10 μg using lysates of mouse brain (S1 fraction).
immunoprecipitation (IP): 5-10 μg using lysates of mouse brain (S1 fraction).
Uniprot accession no.:
产品名称
Anti-EB3 antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~32 kDa
species reactivity
mouse, human, rat
concentration
~1.0 mg/mL
technique(s)
immunoblotting: 1-2 μg/mL using lysates of rat brain (S1 fraction)
immunoprecipitation (IP): 5-10 μg using lysates of mouse brain (S1 fraction).
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MAPRE3(22924)
Application
Anti-EB3 antibody produced in rabbit has been used in:
- immunoblotting
- co-immunoprecipitation
- immunocytochemistry
- glutathione S-transferase (GST) pull-down assay
Biochem/physiol Actions
In neurons, End-binding protein-3 (EB3) has been shown to interact with scaffold protein ankyrin-G and PSD95 and is required for stabilizing neuronal polarity in mature neurons and in determining neuronal dendritic morphology. EB3 is also involved in MT polymerization and cell migration. EB3 may be involved in stabilizing MTs and anchoring them to centrosomes during spindle formation. Knockout of EB3 inhibits myoblasts elongation and fusion into myotubes.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
End-binding protein-3 (EB3), also known as microtubule-associated protein RP/EB 3 (MAPRE3), belongs to a distinct family of “plus-end tracking proteins” (+TIPs). EB proteins are among the most conserved components of the microtubules cytoskeleton. EB proteins contain an N-terminal calponin homology (CH) domain and a unique EB-like C-terminal motif. EB3 is exclusively expressed in the central nervous system and it interacts with a CNS-specific APC homolog APCL.
Immunogen
Synthetic peptide corresponding to an internal sequence of human EB3, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse EB3.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Stim2-Eb3 association and morphology of dendritic spines in hippocampal neurons
Pchitskaya E, et al.
Scientific reports, 7(1), 17625-17625 (2017)
Plus-end-tracking proteins and their interactions at microtubule ends
Galjart N
Current Biology, 20(12), R528-R537 (2010)
PSD-95 alters microtubule dynamics via an association with EB3
Sweet ES, et al.
The Journal of Neuroscience, 31(3), 1038-1047 (2011)
EB3 regulates microtubule dynamics at the cell cortex and is required for myoblast elongation and fusion
Straube and Merdes A
Current Biology, 17(15), 1318-1325 (2007)
EB3, a novel member of the EB1 family preferentially expressed in the central nervous system, binds to a CNS-specific APC homologue
Nakagawa H, et al.
Oncogene, 19(2), 210-210 (2000)
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