产品名称
Monoclonal Anti-Histone H3 (C-terminal) antibody produced in mouse, clone H6.10, purified from hybridoma cell culture
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
H6.10, monoclonal
form
buffered aqueous solution
mol wt
antigen ~14 kDa
species reactivity
chicken, human, monkey, hamster, canine
concentration
~1 mg/mL
technique(s)
immunoblotting: 0.25-0.5 μg/mL using using histones isolated from human HeLa cells.
immunofluorescence: 5-10 μg/mL using using HeLa cells.
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... HIST1H3A(8350)
Application
Monoclonal Anti-Histone H3 (C-terminal) antibody has been used in
- immunoblotting
- immunofluorescence
- western blotting
Biochem/physiol Actions
H3.1 (Histone 3-variant 1) is involved in gene silencing as well as gene activation. Histones H3 and H4 are the predominant histones subjected to extensive covalent modifications. Mutations in histone H3 were identified in various tumors.
Histone cluster 1 H3A (HIST1H3A) has been associated with DNA replication.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Histone cluster 1 H3A (HIST1H3A) belongs to the H3 histone family. The gene encoding it is localized on human chromosome 6.
Monoclonal Anti-Histone H3 (C-terminal) (mouse IgG1 isotype) is derived from the hybridoma H6.10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the C terminal region of human Histone H3, conjugated to KLH.
Immunogen
a synthetic peptide corresponding to a sequence at the C terminal region of human Histone H3.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Histone H3. 3 mutations: a variant path to cancer
Yuen B T K and Knoepfler P S
Cancer Cell, 24(5), 567-574 (2013)
Expression patterns and post-translational modifications associated with mammalian histone H3 variants
Hake S B, et al.
The Journal of Biological Chemistry, 281(1), 559-568 (2006)
Sean M Callahan et al.
Science advances, 9(32), eade2693-eade2693 (2023-08-11)
Histone modifications control numerous processes in eukaryotes, including inflammation. Some bacterial pathogens alter the activity or expression of host-derived factors, including sirtuins, to modify histones and induce responses that promote infection. In this study, we identified a deacetylase encoded by
FERONIA receptor kinase contributes to plant immunity by suppressing jasmonic acid signaling in Arabidopsis thaliana
Guo H, et al
Current Biology, 28(20), 3316-3324 (2018)
Organismal differences in post-translational modifications in histones H3 and H4
Garcia B, et al.
The Journal of Biological Chemistry, 282(10), 7641-7655 (2007)
相关内容
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