SAB4200689
抗-肌动蛋白（α-肌节）抗体，小鼠单克隆
clone 5C5, hybridoma cell culture supernatant
别名:
α-SCA, α-肌节肌动蛋白, a-SCA, 肌动蛋白α肌节/骨骼
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
5C5, monoclonal
Species reactivity:
rat, chicken, bovine, mouse, guinea pig, rabbit, human, planaria(flatworm), fish, Xenopus
Application:
ELISA, IF, IHC
Citations:
7
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
5C5, monoclonal
form
buffered aqueous solution
mol wt
antigen ~42 kDa
species reactivity
rat, chicken, bovine, mouse, guinea pig, rabbit, human, planaria(flatworm), fish, Xenopus
packaging
antibody small pack of 25 μL
technique(s)
ELISA: suitable, immunoblotting: 1:500-1:1000 using rat heart tissue extracts, immunofluorescence: 1:500-1000 using human HeLa cells., immunohistochemistry: 1:500 using formalin-fixed, paraffin-embedded human tongue sections and Biotin/ExtrAvidin®-Peroxidase staining system.
isotype
IgM
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ACTA2(59)
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General description
抗肌动蛋白（肌节）（小鼠IgG1同种型）单克隆抗体来源于小鼠骨髓瘤细胞和纯化的兔横纹肌免疫小鼠的脾细胞融合产生的5C5杂交瘤。肌动蛋白是真核细胞中的主要细胞骨架蛋白。
Immunogen
纯化的兔横纹肌
Application
小鼠抗肌动蛋白(α-肌节)单克隆抗体可用于：
- 间接免疫荧光染色
- 免疫组织化学
- 酶联免疫吸附检测 (ELISA)
- 免疫印迹
- 免疫荧光
Biochem/physiol Actions
肌动蛋白在多种细胞过程中起关键作用，包括细胞迁移、胞质分裂、囊泡转运和收缩力的生成。
Physical form
产物以含有15 mM叠氮化钠作为防腐剂的培养上清液的形式提供。该产品包含牛血清白蛋白和人源蛋白。
Legal Information
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途，或人类或动物任何类型的消费或使用。
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
Na Tang et al.
Nature communications, 13(1), 7455-7455 (2022-12-03)
Intracellular Ca2+ dysregulation is a key marker in septic cardiac dysfunction; however, regulation of the classic Ca2+ regulatory modules cannot successfully abolish this symptom. Here we show that the knockout of transient receptor potential canonical (TRPC) channel isoforms TRPC1 and
PEDF decreases cardiomyocyte edema during oxygen-glucose deprivation and recovery via inhibiting lactate accumulation and expression of AQP1
Huang B, et al.
International Journal of Molecular Medicine, 43(5), 1979-1990 (2019)
The actin cytoskeleton
Molecular and Cellular Biology, 1979-1990 (2000)
Actin depolymerisation and crosslinking join forces with myosin II to contract actin coats on fused secretory vesicles
Miklavc P, et al.
Journal of Cell Science, 128(6), 1193-1203 (2015)
Xinhui Fan et al.
Frontiers in pharmacology, 13, 892643-892643 (2022-07-23)
Diabetes mellitus (DM) often involves cardiovascular complications; however, treatment regimens are limited. ROCK1 (rho-associated coiled-coil containing protein kinase 1) serves as a pathological factor in several diabetic complications. Herein, we aimed to explore the effect of Fasudil (a ROCK1 inhibitor)
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