SAB4200716
Anti-Vimentin antibody, Mouse monoclonal
clone VIM-13.2, purified from hybridoma cell culture
别名:
Anti-F5H288
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
VIM-13.2, monoclonal
Species reactivity:
mouse, bovine, canine, rat, monkey, human, chicken
Application:
IF, IHC
Citations:
7
biological source
mouse
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
VIM-13.2, monoclonal
form
buffered aqueous solution
mol wt
~58 kDa
species reactivity
mouse, bovine, canine, rat, monkey, human, chicken
enhanced validation
independent
Learn more about Antibody Enhanced Validation
concentration
~1.0 mg/mL
technique(s)
immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast HS68 cell line extract, immunofluorescence: 1.25-2.5 μg/mL using human foreskin fibroblast HS68 cells, immunohistochemistry: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
bovine ... Vim(280955)
chicken ... Vim(420519)
dog ... Vim(477991)
human ... VIM(7431)
mouse ... Vim(22352)
rat ... Vim(81818)
rhesus monkey ... Vim(705289)
General description
Anti-Vimentin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the VIM-13.2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with human foreskin fibroblast extract. Vimentin is a type III intermediate filaments (IF) expressed in a wide range of cell types. including, pancreatic precursor cells, fibroblasts and sertoli cells. It is also expressed in trophoblastic giant cells, neuronal precursor cells, lung epithelial cells, renal tubular and stromal cells. Vimentin is also present in leucocytes, macrophages, neutrophils and mesangial cells.
Immunogen
human foreskin fibroblast extract
Application
Anti-Vimentin antibody, Mouse monoclonal has been used in:
- western blotting
- indirect immunofluorescence
- immunohistochemistry
Biochem/physiol Actions
Vimentin plays a significant role in supporting and anchoring the cellular position of organelles, such as nucleus, endoplasmic reticulum, mitochondria (either laterally or terminally). It helps to maintain the mesenchymal cells especially their shape, motility, survival during of mechanical stress. Epithelial cancers like prostate, breast, lung show an increased vimentin expression. Vimentin is also highly expressed in gastrointestinal tumors, central nervous system tumors and malignant melanoma.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
Podoplanin regulates mammary stem cell function and tumorigenesis by potentiating Wntbeta-catenin signaling
Bresson L, et al.
Development, 145(4), dev160382-dev160382 (2018)
Morphological and quantitative analysis on alpha-tubulin modifications in glioblastoma cells
Gadau SD
Neuroscience Letters, 687, 111-118 (2018)
The type III intermediate filament vimentin regulates organelle distribution and modulates autophagy
Biskou O, et al.
Testing, 14(1), e0209665-e0209665 (2019)
Laura Bresson et al.
Development (Cambridge, England), 145(4) (2018-01-24)
Stem cells (SCs) drive mammary development, giving rise postnatally to an epithelial bilayer composed of luminal and basal myoepithelial cells. Dysregulation of SCs is thought to be at the origin of certain breast cancers; however, the molecular identity of SCs
Taito Miyake et al.
Scientific reports, 10(1), 14292-14292 (2020-09-02)
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