SAB4200732
Anti-β-Tubulin I antibody, Mouse monoclonal
clone SAP.4G5, purified from hybridoma cell culture
别名:
Anti-TUBB
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关于此项目
NACRES:
NA.43
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
SAP.4G5, monoclonal
Application:
immunoblotting
immunofluorescence
immunohistochemistry
immunofluorescence
immunohistochemistry
Species reactivity:
bovine, gerbil, Xenopus, human, canine, mouse, hamster, chicken, rat, monkey
Citations:
2
Technique(s):
immunoblotting: 0.06-0.12 μg/mL using HeLa cell extract
immunofluorescence: 1-2.5 μg/mL using HeLa cells
immunohistochemistry: 1-2 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human fallopian tube or testis sections.
immunofluorescence: 1-2.5 μg/mL using HeLa cells
immunohistochemistry: 1-2 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human fallopian tube or testis sections.
Uniprot accession no.:
产品名称
Anti-β-Tubulin I antibody, Mouse monoclonal, clone SAP.4G5, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
SAP.4G5, monoclonal
form
buffered aqueous solution
mol wt
~55 kDa
species reactivity
bovine, gerbil, Xenopus, human, canine, mouse, hamster, chicken, rat, monkey
concentration
~1.0 mg/mL
technique(s)
immunoblotting: 0.06-0.12 μg/mL using HeLa cell extract
immunofluorescence: 1-2.5 μg/mL using HeLa cells
immunohistochemistry: 1-2 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human fallopian tube or testis sections.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TUBB(203068)
Application
Anti-β-Tubulin I antibody, Mouse monoclonal has been used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
Biochem/physiol Actions
β-Tubulin I is also useful in deducing the role that different tubulin isotypes play in nerve development, function or maintenance.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-β-Tubulin I (mouse IgG1 isotype) is derived from the SAP.4G5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. Tubulin is the major building block of microtubules, an intracellular cylindrical filamentous structure, that is present in almost all eukaryotic cells. Tubulin exists in all cells as a 100 kDa protein, which assembles from heterodimers of α-tubulin and β-tubulin; both have a molecular weight of 55 kDa and share considerable homology.
Immunogen
synthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype I, conjugated to BSA
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Tubulin modifications and their cellular functions
Hammond JW, et al.
Current Opinion in Cell Biology, 20(1), 71-76 (2008)
Simon Nicot et al.
Science advances, 9(37), eadi7838-eadi7838 (2023-09-13)
Tubulin posttranslational modifications represent an important mechanism involved in the regulation of microtubule functions. The most widespread among them are detyrosination, α∆2-tubulin, and polyglutamylation. Here, we describe a family of tubulin-modifying enzymes composed of two closely related proteins, KIAA0895L and
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