SAB4200741
Anti-Human IgE antibody, Mouse monoclonal
clone GE-1, purified from hybridoma cell culture
别名:
Anti-Immunoglubulin E
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关于此项目
NACRES:
NA.46
UNSPSC Code:
12352203
Clone:
GE-1, monoclonal
Species reactivity:
human
Application:
ELISA, IP
Citations:
6
biological source
mouse
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
GE-1, monoclonal
form
buffered aqueous solution
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
ELISA: 0.15-0.3 μg/mL using 1 μg/mL Human IgE myeloma for coating, immunoblotting: suitable, immunoprecipitation (IP): 1-2 μg/test using Human IgE myeloma
isotype
IgG2b
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
Anti-Human IgE antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the GE-1 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified human IgE myeloma protein. Immunoglobulin E (IgE) is the least abundant immunoglobulin isotype in circulation. IgE is produced by plasma cells and is highly functional in the lung, skin and mucus membranes. Normal levels of IgE in serum are less than 1 μg/ml.
Immunogen
purified human IgE myeloma protein
Application
Anti-Human IgE antibody, Mouse monoclonal has been used in various immunological techniques, including enzyme linked immunosorbent assay (ELISA), immunoblot, immunoprecipitation and IgE quantification using piezoelectric (Pz) crystal bioassay.
Biochem/physiol Actions
Immunoglobulin E (IgE) plays a critical role in allergic reactions and host protection from parasites. Serum IgE levels are highly elevated during an allergic reaction (such as atopic eczema, extrinsic asthma or hay fever, fungal and parasitic infection, atopic disease or primary immunodeficiency). Detection and measurement of total IgE concentrations is crucial for diagnosis, monitoring and treatment of allergy patients. In addition, the recognition and isolation of allergen-specific IgE can lead to progress in understanding of pathophysiologic mechanisms underlying IgE-associated allergic disorders.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Naohiro Watanabe et al.
Trends in parasitology, 21(4), 175-178 (2005-03-23)
The best-known function of immunoglobulin E (IgE) is the initiation of allergic reaction. However, it does not make sense that IgE exists only for allergy, which is harmful for the body and does not have a beneficial role. In this
D G Ebo et al.
Cytometry. Part B, Clinical cytometry, 72(3), 196-203 (2006-11-18)
Correct identification of the culprit venom is a prerequisite for specific venom immunotherapy (VIT). Despite the efficacy of VIT, issues as how to monitor treatment and when to discontinue maintenance therapy remain to be established. To evaluate diagnostic performances of
Factors affecting human IgE and IgG responses to allergen-like Schistosoma mansoni antigens: Molecular structure and patterns of in vivo exposure
Fitzsimmons CM, et al.
International Archives of Allergy and Immunology, 142, 40-50 (2019)
Immunoglobulin E in health and disease
Amarasekera M, et al.
Asia Pacific Allergy, 1(1), 12-15 (2011)
Precision medicine allergy immunoassay methods for assessing immunoglobulin E sensitization to aeroallergen molecules
Popescu FD, et al.
World journal of methodology, 8, 17-17 (2018)
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