SAB4200760
Anti-Fibronectin Antibody
mouse monoclonal, FN-15
别名:
Anti-CIG, Anti-Cold-insoluble globulin, Anti-FN
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UNSPSC Code:
12352203
NACRES:
NA.41
Clone:
FN-15, monoclonal
Species reactivity:
human, mouse
Application:
immunoblotting
immunofluorescence
immunohistochemistry
immunoprecipitation (IP)
indirect ELISA
immunofluorescence
immunohistochemistry
immunoprecipitation (IP)
indirect ELISA
Technique(s):
immunoblotting: 0.3-0.6 μg/mL using human fetal fibroblast-like WI-38 cell line extract
immunofluorescence: 2.5-5 μg/mL using human foreskin fibroblast Hs68 cells
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: 0.15-0.3 μg/mL using 5 μg/mL Fibronectin from human plasma for coating
immunofluorescence: 2.5-5 μg/mL using human foreskin fibroblast Hs68 cells
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: 0.15-0.3 μg/mL using 5 μg/mL Fibronectin from human plasma for coating
Citations:
2
Uniprot accession no.:
产品名称
Anti-Fibronectin antibody, Mouse monoclonal, clone FN-15, purified from hybridoma cell culture
biological source
mouse
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
FN-15, monoclonal
form
buffered aqueous solution
mol wt
220-260 kDa
species reactivity
human, mouse
concentration
~1 mg/mL
technique(s)
immunoblotting: 0.3-0.6 μg/mL using human fetal fibroblast-like WI-38 cell line extract
immunofluorescence: 2.5-5 μg/mL using human foreskin fibroblast Hs68 cells
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: 0.15-0.3 μg/mL using 5 μg/mL Fibronectin from human plasma for coating
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... FN1(2335)
Application
The antibody may be used in various immunochemical techniques including Immunoblotting (∼220-260kDa)1, ELISA, Immunofluorescence, Immunohistochemistry and Immunoprecipitation.
Biochem/physiol Actions
Anti-Fibronectin antibody, Mouse monoclonal specifically recognizes Fibronectin from plasma and in the pericellular extracellular matrix of cultured fibroblasts from human and mouse origin.
The plasma form of Fibronectin is synthesized by hepatocytes, secreted to blood and, upon tissue injury, is incorporated into fibrin clots to exert effects on platelet function and to mediate hemostasis. Cellular Fibronectin is synthesized by many cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells and myocytes, it is assembled by cells as they migrate into the clot to reconstitute damaged tissue.
General description
Monoclonal Anti-Maltose Binding Protein (MBP) (mouse IgG1 isotype) is derived from the hybridoma MBP-17 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified recombinant MBP fusion protein. Fibronectin (FN), also known as Cold-insoluble globulin (CIG), is a multi-domain glycoprotein composed of two nearly identical disulfide-bound polypeptides. It is widely expressed by multiple cell types and is critically important in vertebrate development, as demonstrated by the early embryonic lethality of mice with targeted inactivation of the FN1 gene 6. In vertebrates, two types of Fibronectin are present: soluble plasma Fibronectin and insoluble cellular Fibronectin. The plasma form of Fibronectin is synthesized by hepatocytes, secreted to blood and, upon tissue injury, is incorporated into fibrin clots to exert effects on platelet function and to mediate hemostasis. Cellular Fibronectin is synthesized by many cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells and myocytes, it is assembled by cells as they migrate into the clot to reconstitute damaged tissue.
Immunogen
Fibronectin isolated from human plasma.
Other Notes
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
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