产品名称
Anti-Tubulin, Tyrosine antibody, Mouse monoclonal, clone TUB-1A2, purified from hybridoma cell culture
biological source
mouse
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
TUB-1A2, monoclonal
form
buffered aqueous solution
mol wt
~55 kDa
species reactivity
Xenopus, Arabidopsis sp., yeast, insect, rat, hamster, porcine, monkey, bovine, human, mouse, canine, chicken
concentration
~1 mg/mL
technique(s)
immunoblotting: suitable
immunofluorescence: 2-4 μg/mL using human HeLa or Chicken fibroblast cells
immunohistochemistry: suitable
isotype
IgG3
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TUBA1A(7846)
Application
Anti-Tubulin, Tyrosine antibody, mouse monoclonal may be used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
Biochem/physiol Actions
Anti-Tubulin, Tyrosine antibody, Mouse monoclonal specifically recognizes the C-terminal Tyrosine of Tubulin protein. The antibody is non-reactive with cells that have been treated with pancreatic carboxypeptidase A under conditions which remove only the C-terminal tyrosine. Monoclonal Anti-Tubulin, Tyrosine recognizes Tubulin from human, mouse, chicken, bovine, rat, hamster, canine, monkey, porcine, arabidopsis, tobacco, yeast, Xenopus, insect, comb jelly, sponge, and worm origin.
Tubulin is the major building block of microtubules which is essential for structural and mobility elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. It is also involved in the nervous system. Tubulin is associated with neurodegenerative disorders such as Alzheimer′s and Parkinson′s disease. Mutations in the αβ- dimers and γ-tubulin are associated with malformation and impairment of cognitive disorders.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Anti-Tubulin, Tyrosine antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the TUB-1A2 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse. Tubulin is an intracellular cylindrical filamentous structure and is presented in eukaryotic cells. It consists of three isoforms α-, β- and γ-tubulin. Tubulin is a heterodimer of α- and β- tubulin subunits.
Immunogen
a synthetic peptide containing the carboxy terminal amino acids of porcine a-tubulin
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Microtubules and resistance to tubulin-binding agents
Kavallaris M
Nature Reviews. Cancer, 10(3), 194-204 (2010)
Tubulin: Structure, Functions and Roles in Disease
Binarova P and Tuszynski J
Cells, 10(3), 194-204 (2019)
相关内容
Instructions
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