SAB4200783
Anti-Human IgG2 antibody, Mouse monoclonal
clone HP-6014, purified from hybridoma cell culture
别名:
Anti-Human immunoglobulin G2
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关于此项目
NACRES:
NA.46
UNSPSC Code:
12352203
Clone:
HP-6014, monoclonal
Species reactivity:
human
Application:
ELISA (i), IF
Citations:
4
isotype
IgG1
biological source
mouse
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HP-6014, monoclonal
form
buffered aqueous solution
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
immunofluorescence: suitable, indirect ELISA: 0.07-0.15 μg/mL using 1 μg/mL Human IgG2 myeloma for coating
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Anti-Human IgG2 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the HP-6014 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgG2 myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads. Human IgG consist of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5, and 4% of the total IgG, respectively.
Immunogen
Purified human IgG2 myeloma protein
Application
Anti-Human IgG2 antibody, Mouse monoclonal may be used in immunological techniques, including enzyme linked immunosorbent assay (ELISA), immunofluorescence, hemagglutination (HA) and hemagglutination inhibition.
Biochem/physiol Actions
Lipopolysaccharides stimulate an IgG2 response in peripheral blood leukocytes (PBL) and an IgG1 response in the spleen. Only IgG1 and IgG3 are capable of adherence to mononuclear phagocytes while IgG2 and IgG4 autoantibodies are much less efficient. The amount of the different IgG subclasses present in the blood shows variation with age. For example, IgG1 and IgG3 reach normal adult levels by 5-7 years of age while IgG2 and IgG4 levels raise more slowly, reaching adult levels at about 10 years of age. Serum IgG subclass deficiencies have been recorded for different patient groups. For example, IgG2 and IgG4 deficiency is found in patients of ataxia telangiectasia. Low IgG2 levels were found in patients with Systemic lupus erythematosus (SLE) and juvenile diabetes mellitus.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Immunoglobulin G; structure and functional implications of different subclass modifications in initiation and resolution of allergy
Scott-Taylor TH, et al.
Immunity, inflammation and disease, 6, 13-33 (2018)
Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood
Blanco E, et al.
The Journal of Allergy and Clinical Immunology, 141(6), 2208-2219 (2018)
Effect of anti-epileptic drugs on serum level of IgG subclasses
Ashrafi MR, et al.
Iranian journal of pediatrics, 20(3), 269-269 (2010)
Immunodeficiency and infections in ataxia-telangiectasia
Nowak-Wegrzyn A, et al.
The Journal of Pediatrics, 144(4), 505-511 (2004)
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