产品名称
抗神经丝200-FITC抗体，小鼠单克隆, clone NE14, purified from hybridoma cell culture
biological source
mouse
conjugate
FITC conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
NE14, monoclonal
form
buffered aqueous solution
mol wt
200 kDa
species reactivity
pig, feline, chicken, bovine, human, mouse, guinea pig, rat
packaging
antibody small pack of 25 μL
concentration
~1 mg/mL
technique(s)
immunohistochemistry: 1:200-1:400 (4-8 ug/mL) using enzyme treated formalin-fixed, paraffin-embedded rat Cerebellum sections
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NEFH(4744)
Biochem/physiol Actions
单克隆抗神经丝200，也称为神经丝-H或重亚基，可特异性识别神经丝200的磷酸化H尾，对酶促去磷酸化的神经丝无反应性。2 该抗体与来自人1、猪1、小鼠3、大鼠4、鸡3、豚鼠3、猫3和牛3的中枢和外周神经系统中的神经丝具有反应性。
神经丝经历翻译后修饰，包括不同水平的磷酸化，这已被建议通过影响神经丝与细胞质细胞器之间的相互作用来调节其功能。神经丝是由三个表观分子量为68（L）、160（M）和200（H）kDa的交织原纤维构成的，原纤维本身由两个单体蛋白四聚体原丝复合物组成。神经丝200也称为神经丝重多肽（H-亚基），NF-H，NEFH或200 kDa神经丝蛋白，在成熟的轴突中具有重要功能，而这两个较小的神经丝蛋白无法保留。
Application
该抗体可用于各种免疫化学技术，包括免疫组织化学和免疫印迹（~200 kDa）。1-7
Disclaimer
除非我们的产品目录另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
神经丝是中间丝（IF）的一种，是支持神经元轴突细胞质的细胞骨架的主要成分。IF是大多数真核细胞的组成部分，并且与细胞的其他细胞骨架元素（即微管和微丝）明显不同。
Immunogen
纯化的猪脊髓神经丝
Physical form
本品为溶于0.01M磷酸盐缓冲液的溶液形式，pH 7.4，含有15 mM叠氮化钠作为防腐剂。
Preparation Note
如需连续使用，可在2-8℃保存长达一个月。若需延长储存时间，可将溶液分装并冷冻。不建议反复冻融。如果长期储存时出现轻微浑浊，请在使用前通过离心澄清溶液。若工作稀释样品在12小时内未使用完，则应丢弃。避免长时间暴露在光线下。
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
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