SAB4200813
Anti-α Actinin antibody, Mouse monoclonal
clone BM-75.2, purified from hybridoma cell culture
别名:
Alpha-actinin cytoskeletal isoform, Alpha-actinin-1, F-actin cross-linking protein, Non-muscle alpha-actinin-1
产品名称
Anti-α Actinin antibody, Mouse monoclonal, clone BM-75.2, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
BM-75.2, monoclonal
mol wt
~100 kDa
species reactivity
human
packaging
antibody small pack of 25 μL
concentration
~1 mg/mL
technique(s)
immunoblotting: 0.5-1 μg/mL using human HeLa cells extract.
immunofluorescence: 5-10 μg/mL using human foreskin fibroblast Hs68 cells.
isotype
IgM
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ACTN1(87)
Application
Anti-α Actinin antibody may be used in various immunochemical techniques including Immunoblot (~100 kDa), Immunohistochemistry and Immunofluorescence. Monoclonal Anti-α-Actinin may be used for immunofluorescent localization of α-actinin in cultured cells and tissues, for immunofluorescent labeling of skeletal muscle (normal and pathological) in order to detect its organizational state, studies of membrane anchorage sites and immunochemical identification of α-actinin by immunoblotting analysis.
Biochem/physiol Actions
α-actinin has extensive proteins association with actin containing stress fibers, in particular with their membrane bound termini.
α-actinin modulates muscle contraction and has a role in cytoskeletal organisation. α-actinin acts as an actin crosslinker and scaffold. Mutations in this gene has been linked to heterogeneous hypertrophic cardiomyopathy and juvenile onset atrial fibrillation.
Monoclonal Anti-α-Actinin specifically recognizes α-Actinin from human1, mouse2, rat3, chicken4, monkey, canine and bovine origin.
Disclaimer
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti- α Actinin (mouse IgM isotype) is derived from the hybridoma BM-72.5 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a cytoskeletal fraction of bovine mammary gland epithelium (BMGE) cultured cells. α-Actinin is an actin binding protein present in both muscle and non-muscle cells. It has a molecular weight of 100,000 daltons and forms dimers in solution. In normal skeletal muscle, α-actinin is associated with the z-discs that define muscle sarcomeres. In smooth muscle, α-actinin has been detected in dense bodies and plaques characteristic of the tissue.
Immunogen
Cytoskeletal fraction of bovine mammary gland epithelium (BMGE) cultured cells.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Preparation Note
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Novel alpha-actinin 2 variant associated with familial hypertrophic cardiomyopathy and juvenile atrial arrhythmias: a massively parallel sequencing study
Girolami F, et al.
Circulation. Cardiovascular Genetics, 7(6), 741-750 (2014)
Feng Zhang et al.
Materials today. Bio, 20, 100626-100626 (2023-05-01)
Heart-on-chip emerged as a potential tool for cardiac tissue engineering, recapitulating key physiological cues in cardiac pathophysiology. Controlled electrical stimulation and the ability to provide directly analyzed functional readouts are essential to evaluate the physiology of cardiac tissues in the
Activation of human neutrophils induces an interaction between the integrin beta 2-subunit (CD18) and the actin binding protein alpha-actinin.
Pavalko FM and LaRoche SM
Journal of immunology (Baltimore, Md. : 1950), 151(7), 3795-3807 (1993)
The vertebrate muscle Z-disc: sarcomere anchor for structure and signalling
Luther PK
Journal of Muscle Research and Cell Motility, 30(5-6), 171-185 (2009)
Molecular mechanics of the α-actinin rod domain: bending, torsional, and extensional behavior.
Golji J, et al.
PLoS Computational Biology, 5(5), e1000389-e1000389 (2009)
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