IgM-purified Isotype Control murine

This mouse IgM monoclonal antibody (clone PFR-03) reacts with undefined epitope on a plant pathogen.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PFR-03, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgM molecule and the respective sample under the particular conditions.

Phytophthora sp.

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Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

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