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Merck
CN
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About This Item

UNSPSC代码:
12352203
NACRES:
NA.26
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重组

expressed in HEK 293 cells

质量水平

方案

>95% (HPLC)
>95% (SDS-PAGE)

表单

lyophilized powder

分子量

25.9 kDa

杂质

<0.1 EU/μg protein (LAL test)

UniProt登记号

运输

wet ice

储存温度

−20°C

基因信息

SARS virus ... S(43740568)

一般描述

Recombinant 2019-nCoV Spike RBD-His Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of 2019-ncov Spike RBD-His (Accession #YP_009724390.1) fused with a 6XHis tag at the C-terminus.

生化/生理作用

Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-COV-2 Spike RBD-His at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-4.53 ng/mL. Immobilized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 36.7 nM as determined in a SPR assay.

制备说明

For lyophilized protein : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8 °C for up to 1 week. This product is stable at ≤ -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Data presented is the available current product information and provided as-is. These products have not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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