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Merck
CN

SAB5900007

Alpaca Anti-MYC single domain antibody, agarose beads

recombinant Alpaca(VHH) Nano antibody agarose bead suspension

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关于此项目

NACRES:
NA.41
Conjugate:
magnetic beads conjugate
Clone:
recombinant monoclonal
Application:
ChIP, IP, RIP, co-IP, mass spectrometry (MS)
Citations:
-
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biological source

alpaca

Quality Segment

recombinant

expressed in Pichia pastoris

conjugate

magnetic beads conjugate

antibody product type

primary antibodies

clone

recombinant monoclonal

form

liquid

technique(s)

ChIP: suitable, RIP: suitable, co-immunoprecipitation (co-IP): suitable, immunoprecipitation (IP): suitable, mass spectrometry (MS): suitable

color

colorless

matrix

(~45 μm (cross-linked to 7.5% magarose beads))

isotype

VHH

capacity

binding capacity(10 μL slurry bind about 2ug-3μg of Myc tagged protein)

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Myc-tag (EQKLISEEDL) has been extensively used as a general epitope tag, which does not generally interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation and purification of the protein of interest. Anti-MYC single domain antibody, agarose beads are agarose beads covalently coupled with VHH antibodies acquiring high specificity and affinity for Myc. These beads can efficiently capture and separate Myc tagged proteins, alongside with the associated proteins from cell extracts of mammal, plant, bacteria, yeast, insect, and other organisms.

Immunogen

MYC Tagged Protein

Biochem/physiol Actions

Selectively recognizes Myc fusion protein

Features and Benefits

  • Anti Tag-Nano antibody beads are single-domain/variable region of heavy chain of camelid antibodies(sdAb/VHH).
  • They are directly coupled to beads covalently.
  • They have unique properties such as small size (~15kDa, 2-4nm) when compared to conventional antibodies.
  • No heavy and light chains in downstream interactions.
  • Acids and alkali resistance, tolerance to harsh conditions.
  • Short incubation time 5 – 30 minutes.
  • Low batch to batch variations.
  • Typically, antibodies are not detectable in Western Blot.

Packaging

plastic tube

Physical form

1XPBS, 0.03% sodium azide, 50% glycerol.

Other Notes

Before usage, please invert the vial several times (DO NOT VORTEX) to form beads suspension, and take out beads’ suspension using a pipette tip with bigger opening (i.e., cut the tip of a 1000 μl pipette tip with sterile scissors). Once opened, it is recommended to use paraffin film to seal the cap.


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存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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