SAB6010029
Anti-Histone H3.3 antibody, clone 4H2, Rabbit Monoclonal
recombinant, expressed in HEK293F cells
别名:
Histone H3.3, H3F3A, H3.3A, H3F3, PP781, AND, H3F3B, H3.3B
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
4H2, recombinant monoclonal
Application:
ELISA, FACS, IF, IHC
Citations:
-
recombinant
expressed in HEK 293 F cells
Quality Segment
conjugate
unconjugated
antibody product type
primary antibodies
clone
4H2, recombinant monoclonal
form
liquid
mol wt
150 kDa
purified by
affinity chromatography
species reactivity
human
concentration
2mg/ml
technique(s)
ELISA: 2000:10000, flow cytometry: 1:50-1:200, immunofluorescence: 1:50-1:200, immunohistochemistry: 1:50-1:200
isotype
IgG
UniProt accession no.
shipped in
wet ice
storage temp.
-10 to -25°C
target post-translational modification
unmodified
General description
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Immunogen
A synthesized peptide derived from human Histone H3.3
Physical form
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preparation Note
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Other Notes
70-100aa
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存储类别
10 - Combustible liquids
WGK
WGK 1
闪点 (F)
Not applicable
闪点 (°C)
Not applicable