产品名称
HRV-3C 蛋白酶, N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL
biological source
human (human Rhinovirus Type 14)
assay
≥90% (SDS-PAGE)
form
aqueous solution
specific activity
≥5000 U/mg
mol wt
21 kDa
concentration
0.8-1.2 mg/mL
technique(s)
protein purification: suitable
suitability
suitable for protein modification
application(s)
life science and biopharma
shipped in
dry ice
storage temp.
−20°C
Quality Level
Physical form
以溶液形式提供，含 50 mM Tris HCl（pH 7.5）、0.15 MNaCl、1 mM TCEP 和 50% (V/V) 甘油。
Other Notes
一单位HRV-3C蛋白酶定义为：在0°C条件下，在反应缓冲液中每小时消化1 nM底物肽（H-Glu-Ala-Leu-Phe-Gln-pNA）所需的酶量。反应缓冲液组成为25 mM HEPES（pH 7.5）、150 mM NaCl、1 mM EDTA和1 mM DTT。
General description
来自人鼻病毒 14 类型的 HRV-3C 蛋白酶是一种在 8 个残基识别序列内特异性切割的蛋白酶。
蛋白水解裂解发生在 Gln 和 Gly 残基之间。
HRV-3C 蛋白酶是一种有用的工具，用于切割在载体结构域和目的蛋白之间以该序列作为融合蛋白表达的重组蛋白。
该重组版本含有 6 个组氨酸标签，可通过 IMAC 色谱法轻松去除。
蛋白水解裂解发生在 Gln 和 Gly 残基之间。
HRV-3C 蛋白酶是一种有用的工具，用于切割在载体结构域和目的蛋白之间以该序列作为融合蛋白表达的重组蛋白。
该重组版本含有 6 个组氨酸标签，可通过 IMAC 色谱法轻松去除。
Application
HRV-3C 蛋白酶已被用于去除 DsCystatin 蛋白上的 GST 标签。
存储类别
10 - Combustible liquids
wgk
WGK 2
法规信息
常规特殊物品
此项目有
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Raheem U et al.
PLoS ONE (2016)
商品
Proteases for biotinylated tag removal for protein purification workflows with related reagents and technical resources.
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