一般描述
Mucinase SmE is a mucin-selective protease that has been engineered to have high activity and and tolerance for site ocupancy and glycoform complexity. Mucinase SmE provides a complementary cleavage profile to Mucinase StcE (Cat No SAE0202). Contrary to StcE digestion that requires an additional tryptic digest prior to MS peptide analysis, when using Mucinase SmE analysis can be done with SmE alone. It is recommended to perform both options to obtain maximal coverage.
应用
Mucinase SmE is useful for glycoproteomics experimentation. It is designed for digestion of densely glycosylated mucin proteins for mass spec based analyses.
特点和优势
- Mucin-selective Protease
- Cleaves N-terminally to O-link glycosylated Ser/Thr in mucin domains
- Complimentary cleavage profile to Mucinase StcE
- Accommodates complex, dense and sialylated glycoforms
- Recombinant with His-Tag®
制备说明
Thaw the Mucinase SmE by brief incubation at 37°C.
The protein solution is stable for 2 weeks at 2-8 °C.
For longer storage, aliquot the protein solution and store at -20 °C.
The enzyme solution should be protected from direct light.
The protein solution is stable for 2 weeks at 2-8 °C.
For longer storage, aliquot the protein solution and store at -20 °C.
The enzyme solution should be protected from direct light.
法律信息
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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