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SBR00036

Rhodamine B labeled Polymyxin B Ready Made Solution

Name: Rhodamine B labeled Polymyxin B Ready Made Solution
Brand: SIGMA

For fluorescent microbial imaging, 1.1 mg/mL in water

别名:

SRB-PMB

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关于此项目

NACRES:

NA.47

UNSPSC Code:

12352211

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属性

Quality Level

100

concentration

1.1 mg/mL in water

lipid type

saturated FAs

storage temp.

−20°C

说明

General description

Rhodamine B labeled polymyxin B (SRB-PMB) is a fluorescent derivative of Polymyxin B. Rhodamine B polymyxin B is an additional fluorescent derivative of Polymyxin B, along with Dansyl labeled polymyxin B (Product # SBR00029). Both products′ mode of action is in accordance with Polymyxin B activity. Fluorescent antibiotics are obtained by synthetic conjugation of an antibiotic to a fluorophore.

Application

Fluorescent antibiotics can be used for many applications including:

Antimicrobial resistance research.
Bacterial visualization and imaging.
Parent antibiotic mode of action research and new antibiotic discovery.
Toxicity studies.
Research of bacterial infections and tracking its uptake in vivo.

Additional possible applications for Rhodamine B labeled Polymyxin B:

Evaluate LPS binding by FRET (fluorescence resonance energy transfer) studies to form a specific complex between fluorescein (FITC)-tagged LPS and Polymyxin B, indicating direct binding of the antibiotic to the LPS
Can be applied as a fluorescent probe to study polymyxin mode of action and its pharmacokinetics
to assess the mitochondrial function of polymyxin B in a kidney cell line (LLC-PK1), suggesting that it changes the mitochondrial membrane polarization.

Analysis Note

Rhodamine B labeled polymyxin B Ready Made Solution is light sensitive.
It is recommended to avoid freeze-thaw cycles of Rhodamine B labeled polymyxin B Ready-Made Solution
Rhodamine B labeled polymyxin B Ready Made Solution (1.1mg/mL) can be diluted 1:100-200 in PBSX1 (Product# D8537) to achieve 0.55-1.1μg/mL final concentration for staining. The above concentration of Rhodamine B labeled polymyxin B was used for Escherichia coli staining (see image)
Fluorescence Microscopy application: Rhodamine B labeled polymyxin B Ready Made Solution excitation (Ex) wavelength is 550-560nm resulting in emission (Em) range of 580-610nm (λ_max=590-600nm)

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

涉药品监管产品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

0000516539

0000491426

0000127004

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Kinetics and Mechanism of the Recognition of Endotoxin by Polymyxin B

Thomas C J, et al.

Journal of the American Chemical Society, 120(48), 12428-12434 (1998)

Action of polymyxin B on bacterial membranes: morphological changes in the cytoplasm and in the outer membrane of Salmonella typhimurium and Escherichia coli B.

P R Schindler et al.

Antimicrobial agents and chemotherapy, 8(1), 95-104 (1975-07-01)

Though the primary action of the cationic antibiotic polymyxin B is against the membrane of susceptible bacteria, severe morphological changes are detected in the cytoplasm. Using fluorescence microscopy and a mono-N-dansyl-polymyxin B derivative, we could demonstrate aggregations of the antibiotic

Pharmacodynamics of polymyxin B against Pseudomonas aeruginosa.

Vincent H Tam et al.

Antimicrobial agents and chemotherapy, 49(9), 3624-3630 (2005-08-30)

Despite limited data, polymyxin B (PB) is increasingly used clinically as the last therapeutic option for multidrug-resistant (MDR) gram-negative bacterial infections. We examined the in vitro pharmacodynamics of PB against four strains of Pseudomonas aeruginosa. Clonal relatedness of the strains

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货号	GTIN
SBR00036-0.5ML	04065266258875