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安全信息

SHC003

Sigma-Aldrich

MISSION® pLKO.1-puro-CMV-TurboGFP™ 阳性对照质粒 DNA

Green fluorescent protein marker to monitor transduction efficiency

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别名:
MISSION® 对照载体
MDL编号:
MFCD07785395
UNSPSC代码:
41106609
NACRES:
NA.51

质量水平

200

产品线

MISSION®

浓度

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

运输

dry ice

储存温度

−20°C

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相关类别

一般描述

MISSION ® TurboGFP ™ 对照载体是一个 8347 碱基对的慢病毒质粒载体，含有编码 TurboGFP ™ 的基因，由 CMV 启动子控制。TurboGFP ™ 对照载体在使用 MISSION ® shRNA 文库克隆的实验中作为阳性转染/转导对照十分有用。 TurboGFP ™ 是从桡足类 Pontellina plumata 中克隆的绿色荧光蛋白 copGFP 的改良变体。

氨苄西林和嘌呤霉素抗生素抗性基因分别在细菌或哺乳动物细胞中提供选择。此外，通过与相容的包装质粒共转染，可在包装细胞 (HEK293T) 中产生自我失活复制无能的病毒颗粒。TurboGFP 对照载体以 10μg 质粒 DNA，溶于 Tris-EDTA (TE) 缓冲液中，浓度为 500 ng/μl.

应用

MISSION® pLKO.1-puro-CMV-TurboGFP ™ 阳性对照质粒 DNA 已被用于构建 GATA2 过表达载体 (oeG2)，方法是在 5′ 端去掉该载体的 GFP 盒。NheI 和 3′PstI 克隆位点，并用全长 GATA2 转录序列替代。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna

法律信息

使用本产品需遵守一个或多个许可协议。有关详细信息，请参阅http://sigmaaldrich.com/missionlicense。
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboBeads is a trademark of TurboBeads LLC
TurboGFP is a trademark of Evrogen Co.

推荐

产品编号
说明
价格

SHC002

任务 ® pLKO.1-puro 非哺乳动物 shRNA 对照质粒 DNA, Targets no known mammalian genes

SHC001V

MISSION®pLKO.1-puro空载体对照转导颗粒, Contains no shRNA insert

SHC002V

MISSION®, Targets no known mammalian genes

SHC003V

MISSION®, Green fluorescent protein marker to monitor transduction efficiency

SHC004

MISSION® 对照载体, shRNA sequence targeting tGFP

SHC004V

MISSION® TurboGFP™ shRNA Control Transduction Particles, shRNA sequence targeting tGFP

SHM01

ExpressMag® Super Magnetic Kit, Increases transduction efficiency

SHM02

ExpressMag® 96-Well Magnetic Kit, Increases transduction efficiency

SHC012

MISSION® 对照载体, Contains a gene encoding TagRFP

SHC014

MISSION® 对照载体, Contains a gene encoding TurboGFP, under the control of the UbC promoter

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What competent cells can I use to propagate the MISSION® Control Vector Purified DNA TurboGFP™ plasmid, Product SHC003?

    We use GC5 cells (identical genotype to DH5α-T1R) to propagate the Mission shRNA plasmids and controls.

  4. How much plasmid DNA do I receive when I order MISSION® Control Vector Purified DNA TurboGFP™, Product SHC003?

    Each MISSION Control Vector is provided as 10 μg purified plasmid DNA at a concentration of ~500 ng/μL in 10 mM Tris-HCl, pH 8.0, containing 1 mM EDTA.

  5. Can I use MISSION® Control Vector Purified DNA TurboGFP™ plasmid, Product SHC003, to produce lentiviral particles?

    The MISSION® Lentiviral Packaging Mix can be used if you wish to produce virus in your own lab. The product code is SHP001. This product is an optimized formulation of two plasmids expressing the key HIV packaging genes and a heterologous viral envelope gene. The Lentiviral Packaging Mix is designed to be co-transfected along with a compatible lentiviral transfer vector (such as SHC003) into HEK293T cells in order to create high-titer pseudo-typed lentiviral particles used for downstream transduction applications. The procedure is available in the Technical Bulletin for product SHP001.

  6. Can I purchase lentiviral particles produced using MISSION® Control Vector Purified DNA TurboGFP™ plasmid, Product SHC003, directly from Sigma-Aldrich?

     Yes. The product code is SHC003V.

  7. What other MISSION® shRNA controls are available from Sigma-Aldrich?

    A complete list of our MISSION® controls can be found here.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Hoekstra AS
Oncotarget, 6(36), 38777-38788 (2015)
Selma Zargari et al.
BMC cancer, 20(1), 789-789 (2020-08-21)
MEIS1 (Myeloid ecotropic viral integration site 1) as a homeobox (HOX) transcription factor plays regulatory roles in a variety of cellular processes including development, differentiation, survival, apoptosis and hematopoiesis, as well as stem cell regulation. Few studies have established pluripotency
Chemical Inhibition of Histone Deacetylases 1 and 2 Induces Fetal Hemoglobin through Activation of GATA2.
Shearstone JR
PLoS ONE, 11(4) (2016)
Min Joo Kim et al.
Scientific reports, 9(1), 13288-13288 (2019-09-19)
Although various chemokines have pro-tumorigenic actions in cancers, the effects of CXCL16 remain controversial. The aim of this study was to investigate the molecular characteristics of CXCL16-expressing papillary thyroid cancers (PTCs). CXCL16 expressions were significantly higher in PTCs than benign
Reihaneh Alsadat Mahmoudian et al.
Molecular genetics & genomic medicine, 7(7), e00746-e00746 (2019-05-16)
MEIS1 (Myeloid ecotropic viral integration site 1), as a homeobox (HOX) transcription factor, has a dual function in different types of cancer. Although numerous roles are proposed for MEIS1 in differentiation, stem cell function, gastrointestinal development and tumorigenesis, the involved
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shRNA Process and Diagram

When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.

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