Merck
CN

SHC012

Sigma-Aldrich

MISSION® 对照载体

Contains a gene encoding TagRFP

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别名:
MISSION® Control Vectors
MDL编号:
NACRES:
NA.51

质量水平

产品线

MISSION®

浓度

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

运输

dry ice

储存温度

−20°C

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一般描述

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, we recommend producing self-inactivating replication incompetent viral particles in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The CMV-TagRFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

应用

To see more application data, protocols, vector maps visit sigma.com/shrna.
The MISSION TagRFP Control Vector is an 8594 base pair lentivirus plasmid vector that contains a gene encoding TagRFP, under the control of the CMV promoter. The CMV-TagRFP Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones.

法律信息

使用本产品需遵守一个或多个许可协议。有关详细信息,请参阅http://sigmaaldrich.com/missionlicense
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TagRFP is a trademark of Evrogen Co.

储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Chi Huu Nguyen et al.
Scientific reports, 9(1), 9139-9139 (2019-06-27)
Acute myeloid leukemia (AML) is a heterogeneous disease with respect to its genetic and molecular basis and to patients´ outcome. Clinical, cytogenetic, and mutational data are used to classify patients into risk groups with different survival, however, within-group heterogeneity is

商品

When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.

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