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Merck
CN

SHC204

Sigma-Aldrich

MISSION® 对照载体

shRNA sequence targeting tGFP

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别名:
MISSION® Control Vectors
MDL编号:
UNSPSC代码:
41106609
NACRES:
NA.51

质量水平

100
200

产品线

MISSION®

浓度

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

运输

dry ice

储存温度

−20°C

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一般描述

The MISSION® TRC2 Control Vector pLKO-puro TurboGFP shRNA is a 7518 base pair lentivirus plasmid vector that contains an shRNA sequence targeting TurboGFP. This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE. The TurboGFP shRNA Control Vector is useful as a positive knockdown control in experiments using the MISSION® TurboGFP positive control vector in cell lines expressing TurboGFP. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (SHP001). The TRC2 TurboGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

应用

Small interfering RNAs (siRNAs) expressed from short hairpin RNAs (shRNAs) are a powerful way to mediate gene specific RNA interference (RNAi) in mammalian cells. The MISSION product line is based on a viral vector-based RNAi library against annotated mouse and human genes. shRNAs that generate siRNAs intracellularly are expressed from amphotropic lentivirus viral particles, allowing screening in a wide range of mammalian cell lines. In these cell lines, MISSION shRNA clones permit rapid, cost efficient loss-of-function and genetic interaction screens.
To see more application data, protocols, vector maps visit sigma.com/shrna.

法律信息

使用本产品需遵守一个或多个许可协议。有关详细信息,请参阅http://sigmaaldrich.com/missionlicense
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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