推荐产品
质量水平
100
200
产品线
MISSION®
浓度
≥1x106 VP/ml (via p24 assay)
运输
dry ice
储存温度
−70°C
一般描述
The MISSION® TRC2 pLKO.5-puro Non-Target shRNA Control Tranduction Particles contain an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION® shRNA library clones. This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Transduction Particles are provided as 200 uL at 1 x 106 TU/mL via p24 assay.
当使用MISSION® TRC shRNA克隆进行实验时,选择适当对照品是您的实验设计的关键要素,以便准确解释敲低结果。 MISSION对照转导颗粒是监测转导效率的关键阳性对照。
想要查看更多应用数据、实验方案和载体图谱,请访问 sigma.com/shrna。
想要查看更多应用数据、实验方案和载体图谱,请访问 sigma.com/shrna。
应用
To see more application data, protocols, vector maps visit sigma.com/shrna.
法律信息
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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