一般描述
我们全面的shRNA产品系列包括超过150,000个预克隆的shRNA构建体，靶向超过15,000个人类基因和15,000个小鼠基因。该文库可用于：基因家族集合：与特定功能类（如激酶和离子通道）相关的基因集合；基因靶标集合，针对靶标基因的shRNA克隆集合；以及单个shRNA克隆。我们的shRNA和慢病毒制造平台利用专门实验室，配备最先进的机器人技术、液体处理和实验室信息管理系统（LIMS），为您的RNAi研究需求提供卓越的质量和服务。
MISSION® TRC shRNA纯化质粒DNA形式省去了从每个构建体制备DNA的复杂操作。DNA可以直接用于瞬时或稳定转染。 另外，这些质粒可用于在包装细胞系中与包装质粒一起产生慢病毒颗粒。为靶标组中的每个构建体提供1μg DNA。
应用
MISSION® shRNA已被用于：
- ZO-1（胞质紧密粘连蛋白1）敲低
- 在等基因MCF10模型中敲低LIF（白血病抑制因子）表达
- 在n MIN6胰腺β细胞中敲低红细胞衍生核因子2样蛋白1
其他说明
想要查看实验方案和应用数据，请访问 sigma.com/shrna。
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Deficiency of long isoforms of Nfe2l1 sensitizes MIN6 pancreatic beta cells to arsenite-induced cytotoxicity
Cui Q, et al.
Toxicology and Applied Pharmacology, 329(2), 67-74 (2017)
Stage-specific roles of FGF2 signaling in human neural development
Grabiec M, et al.
Stem Cell Research, 17(2), 330-341 (2016)
Epigenetic up-regulation of leukemia inhibitory factor (LIF) gene during the progression to breast cancer
Shin JE, et al.
Molecules and Cells, 31(2), 181-189 (2011)
Emily L Morton et al.
Cell reports, 27(1), 154-171 (2019-04-04)
Transcriptional circuit architectures in several organisms have been evolutionarily selected to dictate precise given responses. Unlike these cellular systems, HIV is regulated through a complex circuit composed of two successive phases (host and viral), which create a positive feedback loop
Harry G Sherman et al.
Biochimica et biophysica acta. Bioenergetics, 1860(8), 628-639 (2019-06-24)
Trans-plasma membrane electron transfer (tMPET) is a process by which reducing equivalents, either electrons or reductants like ascorbic acid, are exported to the extracellular environment by the cell. TPMET is involved in a number of physiological process and has been
商品
MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.
RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.
相关内容
寻找单独基因的shRNA超过150,000个预克隆的shRNA构建体，靶向超过15,000个人类基因和15,000个小鼠基因。
Find shRNAs for Individual Genes. Over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes.
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