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Merck
CN

SHCLNV

Sigma-Aldrich

MISSION® shRNA

Lentiviral Particles

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UNSPSC代码:
41106609

主要文件

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质量水平

200

技术

capture ELISA: 106 VP/mL using p24

运输

dry ice

储存温度

−70°C

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一般描述

我们全面的shRNA产品系列包括超过150,000个预克隆的shRNA构建体，靶向超过15,000个人类基因和15,000个小鼠基因。该文库可用于：基因家族集合：与特定功能类（如激酶和离子通道）相关的基因集合；基因靶标集合，针对靶标基因的shRNA克隆集合；以及单个shRNA克隆。我们的shRNA和慢病毒制造平台利用专门实验室，配备最先进的机器人技术、液体处理和实验室信息管理系统（LIMS），为您的RNAi研究需求提供卓越的质量和服务。
慢病毒颗粒广泛用于转导各种细胞系。MISSION®慢病毒颗粒含有水疱性口炎病毒(VSV-G)假型，可转导细胞范围更大。 此外，该慢病毒颗粒可将shRNA稳定整合到分裂和非分裂细胞的基因组中。 MISSION生物技术团队稳定产出高质量慢病毒，让我们的客户可以直接开展RNAi实验，不用再将宝贵的时间浪费在繁琐的慢病毒制备上。 我们最高的滴度是106 VP/mL。 不同于腺病毒转染需要高度过量病毒，慢病毒转染更为高效，MOI值（感染复数）低至1。

应用

MISSION® shRNA已用于：
  • 转染和转导
  • 构建表达shRNA的稳定细胞系
  • 建立α-辅肌动蛋白-4(ACTN4)敲低细胞系
  • 转导RAW 264.7细胞

其他说明

想要查看实验方案和应用数据，请访问 sigma.com/shrna。

法律信息

使用本产品需遵守一个或多个 许可协议。
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

高风险级别生物产品--病毒，疫苗等
低风险生物材料
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Bile acids reduce endocytosis of high-density lipoprotein (HDL) in HepG2 cells.
Rohrl C, Eigner K, Fruhwurth S, et al.
PLoS ONE, 9(7), e102026-e102026 (2014)
alpha-Actinin-4 confers radioresistance coupled invasiveness in breast cancer cells through AKT pathway
Desai S, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1865(1), 196-208 (2018)
Xia Liu et al.
Oncology letters, 16(5), 5868-5874 (2018-10-20)
Ovarian cancer (OC) has the highest fatality rates of all gynecological malignancies worldwide. The epithelial-to-mesenchymal transition (EMT) serves an essential role in the progression of OC. An improved understanding of the molecular mechanism underlying EMT in OC may increase the
Sejal Desai et al.
Biochimica et biophysica acta, 1865(1), 196-208 (2017-10-23)
Acquired radioresistance accompanied with increased metastatic potential is a major hurdle in effective radiotherapy of breast cancers. However, the nature of their inter-dependence and the underlying mechanism remains largely intangible. By employing radioresistant (RR) cell lines, we herein demonstrate that
Vera Mugoni et al.
Cell research, 29(6), 446-459 (2019-04-27)
Although targeted therapies have proven effective and even curative in human leukaemia, resistance often ensues. IDH enzymes are mutated in ~20% of human AML, with targeted therapies under clinical evaluation. We here characterize leukaemia evolution from mutant IDH2 (mIDH2)-dependence to
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商品

Safe Lentivirus Handling

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

MISSION® Lentiviral shRNA

MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.

Efficient Gene Silencing with MISSION® TRC shRNA

RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.

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实验方案

CRISPR Lentiviral Screening: Antibiotic Selection

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

相关内容

shRNAs for Individual Genes

Find shRNAs for Individual Genes. Over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes.

单独基因的shRNA

寻找单独基因的shRNA超过150,000个预克隆的shRNA构建体，靶向超过15,000个人类基因和15,000个小鼠基因。

(SHCLNV) - Technical Bulletin

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