跳转至内容
Merck
CN

SHCLNV

MISSION® shRNA

Lentiviral Particles

登录 查看组织和合同定价。

关于此项目

UNSPSC Code:
41106609

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

MISSION® shRNA, Lentiviral Particles

technique(s)

capture ELISA: 106 VP/mL using p24

shipped in

dry ice

storage temp.

−70°C

Quality Level

200

正在寻找类似产品? 访问 产品对比指南

相关类别

Legal Information

使用本产品需遵守一个或多个 许可协议。
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

想要查看实验方案和应用数据，请访问 sigma.com/shrna。

Application

MISSION® shRNA已用于：
  • 转染和转导
  • 构建表达shRNA的稳定细胞系
  • 建立α-辅肌动蛋白-4(ACTN4)敲低细胞系
  • 转导RAW 264.7细胞

General description

我们全面的shRNA产品系列包括超过150,000个预克隆的shRNA构建体，靶向超过15,000个人类基因和15,000个小鼠基因。该文库可用于：基因家族集合：与特定功能类（如激酶和离子通道）相关的基因集合；基因靶标集合，针对靶标基因的shRNA克隆集合；以及单个shRNA克隆。我们的shRNA和慢病毒制造平台利用专门实验室，配备最先进的机器人技术、液体处理和实验室信息管理系统（LIMS），为您的RNAi研究需求提供卓越的质量和服务。
慢病毒颗粒广泛用于转导各种细胞系。MISSION®慢病毒颗粒含有水疱性口炎病毒(VSV-G)假型，可转导细胞范围更大。 此外，该慢病毒颗粒可将shRNA稳定整合到分裂和非分裂细胞的基因组中。 MISSION生物技术团队稳定产出高质量慢病毒，让我们的客户可以直接开展RNAi实验，不用再将宝贵的时间浪费在繁琐的慢病毒制备上。 我们最高的滴度是106 VP/mL。 不同于腺病毒转染需要高度过量病毒，慢病毒转染更为高效，MOI值（感染复数）低至1。

存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

高风险级别生物产品--病毒，疫苗等
低风险生物材料
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 文件 section.

如需帮助，请联系 客户支持

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Barbara D Boyan et al.
Scientific reports, 8(1), 8588-8588 (2018-06-07)
Successful osseointegration of an endosseous implant involves migration and differentiation of mesenchymal stem cells (MSCs) on the implant surface. Micro-structured, hydrophilic titanium surfaces direct MSCs to undergo osteoblastic differentiation in vitro, in the absence of media additives commonly used in
Vera Mugoni et al.
Cell research, 29(6), 446-459 (2019-04-27)
Although targeted therapies have proven effective and even curative in human leukaemia, resistance often ensues. IDH enzymes are mutated in ~20% of human AML, with targeted therapies under clinical evaluation. We here characterize leukaemia evolution from mutant IDH2 (mIDH2)-dependence to
Sejal Desai et al.
Biochimica et biophysica acta, 1865(1), 196-208 (2017-10-23)
Acquired radioresistance accompanied with increased metastatic potential is a major hurdle in effective radiotherapy of breast cancers. However, the nature of their inter-dependence and the underlying mechanism remains largely intangible. By employing radioresistant (RR) cell lines, we herein demonstrate that
Inhibitors of endoplasmic reticulum alpha-glucosidases potently suppress hepatitis C virus virion assembly and release
Qu X, et al.
Antimicrobial agents and chemotherapy, 55(3), 1036-1044 (2011)
Rene Olivares-Navarrete et al.
PloS one, 12(1), e0170312-e0170312 (2017-01-18)
Stem cell fate has been linked to the mechanical properties of their underlying substrate, affecting mechanoreceptors and ultimately leading to downstream biological response. Studies have used polymers to mimic the stiffness of extracellular matrix as well as of individual tissues
查看所有相关文献

商品

Safe Lentivirus Handling

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

MISSION® Lentiviral shRNA

MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.

Efficient Gene Silencing with MISSION® TRC shRNA

RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.

查看所有结果

实验方案

CRISPR Lentiviral Screening: Antibiotic Selection

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

相关内容

shRNAs for Individual Genes

Find shRNAs for Individual Genes. Over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes.

单独基因的shRNA

寻找单独基因的shRNA超过150,000个预克隆的shRNA构建体，靶向超过15,000个人类基因和15,000个小鼠基因。

Bulletin - SHCLNV

Instructions

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持