SML3448
Propidium monoazide
≥98% (HPLC), Fluorescent DNA cross-linker, powder
别名:
3-Amino-8-azido-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridium dichloride, Propidium monoazide dichloride
生化/生理作用
Propidium derivative that allows light-induced crosslinkage to DNA for highly selective live-dead cells analysis by PCR or fluorescence microscopy.
Propidium monoazide (PMA) is a propidium derivative with an azide group that allows crosslinkage to DNA upon light exposure. PMA is only permeable to bacteria with compromised membrane integrity, making it highly selective for sick/dying/dead cells over healthy cells. PMA is typically added to cell samples (50 μM for 1-15 min in the dark) prior to cross-linking induction (650-W halogen light source), washing and DNA extraction. Due to its higher charge, PMA (+2) is less permeant to live cells and more selective than ethidium monoazide/EMA (+1) for dead cells. The stained cells can also be viewed and analyzed by fluorescence microscopy.
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
First Report on the Rapid Detection and Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) in Viable but Non-culturable (VBNC) Under Food Storage Conditions
Frontiers in Microbiology, 11, 615875-615875 (2021)
Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells
Journal of Microbiological Methods, 67(2), 310-320 (2006)
Listeria monocytogenes Assessment in a Ready-to-Eat Salad Shelf-Life Study Using Conventional Culture-Based Methods, Genetic Profiling, and Propidium Monoazide Quantitative PCR
Foods (Basel, Switzerland), 10(2), 235-235 (2021)
Detection and Potential Virulence of Viable but Non-Culturable (VBNC) Listeria monocytogenes: A Review
Microorganisms, 9(1), 194-194 (2021)
Rapid profiling of drug-resistant bacteria using DNA-binding dyes and a nanopore-based DNA sequencer
Scientific Reports, 11(1), 3436-3436 (2021)
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