HMSiR680-Me

HMSiR680-Me is a cell-permeable silicon rhodamine (SiR) far-red fluorophore that selectively illuminates acidic organelles, such as lysosomes, in live cells, owing to its unique pH-dependent fluorescence. It is ideal for complex imaging experiments, offering high photostability and minimal impact on cell health or organelle motility. HMSiR680-Me enables multicolor imaging alongside other dyes, with protocols for crosstalk calculation and spectral unmixing to ensure clear and distinct signals. Its utility extends to various applications, including overnight imaging and super-resolution microscopy, making HMSiR680-Me a valuable tool for studying cellular processes and diseases related to lysosomal function. Ex: ~ 680 nm & Em: ~ 695 nm; Detection window ~ 690-830 nm; pKcyclic = 6.2.