Iodoacetamide-alkyne-C6

Iodoacetamide-alkyne C6 (IAA-C6, IA-alkyne-C6, IAM-alkyne-C6) is a bifunctional thiol-reactive probe designed for activity-based protein profiling (ABPP) of reactive cysteine residues in live cells. IA-alkyne probe combines aniodoacetamide group (covalent warhead) for cysteine labeling with an alkynemoiety that serves as a click-chemistry handle, enabling subsequent attachmentof fluorophores or biotin-linked reporter for visualization or enrichment, respectively. Optimal usage concentrations are ≤3 μM for cellular studies and ~100 μM for lysates and tissue homogenates, facilitating comprehensive proteomic analysisof cysteine-dependent enzyme activities and protein functions.

Iodoacetamide-alkyne-C6may be used as a chemical probe for labeling cysteine residues in proteins,allowing for the attachment of fluorophores or biotin for visualization orenrichment in proteomic studies.

Cysteine-reactive ABPP probe enabling live-cell labeling, visualization, and enrichment of thiol-containing proteins.