Quality Segment
assay
≥98% (HPLC)
form
powder
color
white to beige
solubility
DMSO: 2 mg/mL, clear
storage temp.
-10 to -25°C
SMILES string
O=C(N1CCC(NCCOCCOCCNC(CCCC[C@H]2[C@@]3([C@]([H])(CS2)NC(N3)=O)[H])=O)=O)C=CC1=O
General description
Maleimide-PEG2-biotin, a maleimide-conjugated biotin molecule with a medium-length spacer arm (PEG)2, is membrane-impermeable and exhibits high affinity for free thiol groups on cysteines.
Application
Maleimide-PEG2-biotin is suitable for use in labeling and purifying biomolecules with high specificity. It may be used in mass spectrometry to detect free sulfhydryl residues in intact proteins.
Biochem/physiol Actions
Biotin-PEG2-Mal, a biotin maleimide linker, forms stable thioether bonds by covalently labeling cysteine sulfhydryl groups on antibodies or proteins and can be detected via streptavidin-HRP in Western blot assays following pull-down. Maleimide labels react with sulfhydryl (–SH) groups at acidic pH, minimizing the risk of disulfide bond scrambling during labeling that can happen at higher pH levels.
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable