SML4178
Maleimide-PEG2-biotin
≥98% (HPLC), powder, biotinylation reagent
别名:
(3aS,4S,6aR)-N-[2-[2-[2-[[3-(2,5-Dihydro-2,5-dioxo-1H-pyrrol-1-yl)-1-oxopropyl]amino]ethoxy]ethoxy]ethyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide, Biotin-PEG2-Mal, Biotin-PEO-maleimide
质量水平
方案
≥98% (HPLC)
表单
powder
颜色
white to beige
溶解性
DMSO: 2 mg/mL, clear
储存温度
-10 to -25°C
SMILES字符串
O=C(N1CCC(NCCOCCOCCNC(CCCC[C@H]2[C@@]3([C@]([H])(CS2)NC(N3)=O)[H])=O)=O)C=CC1=O
一般描述
Maleimide-PEG2-biotin, a maleimide-conjugated biotin molecule with a medium-length spacer arm (PEG)2, is membrane-impermeable and exhibits high affinity for free thiol groups on cysteines.
应用
Maleimide-PEG2-biotin is suitable for use in labeling and purifying biomolecules with high specificity. It may be used in mass spectrometry to detect free sulfhydryl residues in intact proteins.
生化/生理作用
Biotin-PEG2-Mal, a biotin maleimide linker, forms stable thioether bonds by covalently labeling cysteine sulfhydryl groups on antibodies or proteins and can be detected via streptavidin-HRP in Western blot assays following pull-down. Maleimide labels react with sulfhydryl (–SH) groups at acidic pH, minimizing the risk of disulfide bond scrambling during labeling that can happen at higher pH levels.
法规信息
新产品
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历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Mitchell R O'Connell et al.
Nature, 516(7530), 263-266 (2014-10-03)
The CRISPR-associated protein Cas9 is an RNA-guided DNA endonuclease that uses RNA-DNA complementarity to identify target sites for sequence-specific double-stranded DNA (dsDNA) cleavage. In its native context, Cas9 acts on DNA substrates exclusively because both binding and catalysis require recognition
Brendan Farrell et al.
Nature, 625(7995), 578-584 (2023-12-21)
The symptoms of malaria occur during the blood stage of infection, when parasites invade and replicate within human erythrocytes. The PfPCRCR complex1, containing PfRH5 (refs. 2,3), PfCyRPA, PfRIPR, PfCSS and PfPTRAMP, is essential for erythrocyte invasion by the deadliest human
Aki Nakayama et al.
Rinsho byori. The Japanese journal of clinical pathology, 59(11), 1013-1018 (2012-02-23)
We examined the relationship between oxidative damage and molecular instability of urinary albumin in the urine of patients with IgA nephropathy (IgAN). As measure of oxidation, we detected the free thiol group at Cys34 in albumin (albumin-Cys34) using maleimide-PEG2-biotin reagent;
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