Desthiobiotin polyethyleneoxide iodoacetamide

Desthiobiotin polyethyleneoxide iodoacetamide (IA-DTB) is a precision chemical probe for activity-based protein profiling (ABPP) that excels in sophisticated systems, from the exofacial proteome of living cells to clinically relevant primary human cells. Its utility expands beyond quantifying reactive cysteines to mapping dynamic post-translational modifications (PTMs) like S-nitrosylation. The probe′s trifunctional architecture—a specific iodoacetamide warhead, a hydrophilic polyethyleneoxide linker, and a desthiobiotin tag—is optimized for enrichment and mass spectrometry-based identification. Its strategic value is fully realized in competitive profiling experiments, where the desthiobiotin tag′s moderate affinity for streptavidin allows for mild elution and maximal recovery of captured biomolecules. This feature makes it a cornerstone tool for defining the ′ligandable proteome′ and deconvoluting the mechanism of action for covalent therapeutics in physiologically relevant systems.

Hygroscopic, Store with desiccant