5-TAMRA Azide

5-TAMRA Azide is a photostable, azide-functionalized carboxy-tetramethylrhodamine reporter that enables specific covalent labeling of alkyne-tagged biomolecules via bioorthogonal click chemistry. Its utility spans both copper-catalyzed (CuAAC) and copper-free strain-promoted (SPAAC) cycloadditions, facilitating applications from fixed-sample proteomics to real-time live-cell imaging. The probe leverages a bright TAMRA fluorophore (λex/λem ≈ 546/579 nm; ε ≈ 91,000 M⁻¹cm⁻¹; Φ ≈ 0.4) that maintains stable fluorescence from pH 4–9, ensuring robust quantification in fluorescence microscopy, flow cytometry, and in-gel scanning. Its pivotal advantage is the fusion of high-fidelity bioorthogonal reactivity with an orange-red spectral profile ideal for multiplexed analysis, minimizing interference from cellular autofluorescence and crosstalk with common blue and green fluorescent channels.

Hygroscopic, Store with desiccant