Merck
CN

SRE0019

Sigma-Aldrich

三磷酸腺苷双磷酸酶 来源于马铃薯

Suitable for manufacturing of diagnostic kits and reagents, ATPase ≥200 units/mg protein

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别名:
腺苷5′-三磷酸酶, 腺苷5′-二磷酸酶
CAS号:
EC 号:
MDL编号:
NACRES:
NA.32

质量水平

类型

Grade VII

形式

lyophilized powder

ATPase activity

≥200 units/mg protein

secondary activity

≥50 % of base activity ADPase

组成

Protein, 25-60%

溶解性

H2O: soluble 1.0 mg/mL

应用

diagnostic assay manufacturing

异质活性

Acid Phosphatase ≤2% of base activity

储存温度

−20°C

应用

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation.
不同种类的马铃薯(S. tuberosum)中存在至少2种同工酶:一种的ATP酶/ADP酶比例较高(∼10)和另一种较低(∼1)。
反应:ATP → ADP+Pi → AMP+2Pi

生化/生理作用

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

单位定义

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

外形

Partially purified, lyophilized powder containing potassium succinate buffer salts.

制备说明

按 ATP 酶单位销售。

其他说明

该等级具有低 ATP 酶/ADP 酶比率。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Kettlun, A., et al.
Phytochemistry, 21, 551-551 (1982)
Differentiation of platelet-aggregating effects of human tumor cell lines based on inhibition studies with apyrase, hirudin, and phospholipase.
E Bastida et al.
Cancer research, 42(11), 4348-4352 (1982-11-01)
David Riewe et al.
Plant physiology, 147(3), 1092-1109 (2008-05-16)
Apyrases hydrolyze nucleoside triphosphates and diphosphates and are found in all eukaryotes and a few prokaryotes. Although their enzymatic properties have been well characterized, relatively little is known regarding their subcellular localization and physiological function in plants. In this study

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