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关于此项目
化学文摘社编号:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-668-6
MDL number:
Specific activity:
≥250 units/mg solid (using pyrogallol)
SMILES string
[O+H2]O[O-]
InChI
1S/H2O3/c1-3-2/h1-2H
InChI key
JSPLKZUTYZBBKA-UHFFFAOYSA-N
type
Type VI
form
powder, stabilized
specific activity
≥250 units/mg solid (using pyrogallol)
technique(s)
ELISA: suitable, immunoassay: suitable, lateral flow assay: suitable, western blot: suitable
color
brown
solubility
phosphate buffer: soluble
suitability
suitable for immunoassay, suitable for manufacturing use
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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General description
辣根过氧化物酶(HRP)分离自辣根(Amoracia rusticana)。HRP是含有四个二硫键的单链多肽。其分子量(约44 kDa)包括多肽链(33,890道尔顿)、氯化血红素加Ca2+(约700道尔顿)和碳水化合物(约9,400道尔顿)。至少存在7种HRP同工酶。辣根过氧化物酶同工酶的等电点范围为3.0-9.0。
辣根过氧化物酶是从辣根(Amoracia rusticana)中分离出来的,属于过氧化物酶的铁原卟啉基团。HRP是含有四个二硫键的单链多肽。它是一种含有18%碳水化合物的糖蛋白。碳水化合物由半乳糖、阿拉伯糖、木糖、岩藻糖、甘露糖、甘露糖胺和半乳糖胺组成,具体取决于特定同工酶。其分子量(约44 kDa)包括多肽链(33,890道尔顿)、氯化血红素加Ca2+ (约700道尔顿)和碳水化合物(约9,400道尔顿)。至少存在7种HRP同工酶。 辣根过氧化物酶同工酶的等电点范围为3.0-9.0。
Application
HRP用于各种免疫测定,如ELISA、蛋白质印迹法(WB)和侧向层析检测。HRP适于学术研究、诊断和药物研究。更多应用请参阅下列技术文章:辣根过氧化物酶(HRP)简介及应用。
来自辣根的过氧化物酶已用于免疫印迹。
Analysis Note
RZ(Reinheitszahl)是指溶于去离子水中的酶(0.5–1.0 mg/ml)的吸光度比值A403/A275 。 它是血红素含量的量度,而不是酶活性。 即使是RZ值较高的制剂也可能具有较低的酶活性。
Other Notes
一个连苯三酚单元将在20℃、pH 6.0条件下,在20秒内从连苯三酚形成1.0mg的红棓酚。
有关过氧化物酶的更多信息,请访问 www.sigma-aldrich.com/enzymeexplorer。
Legal Information
M-CLARITY is a trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
法规信息
低风险生物材料
此项目有
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Sun Y, et al.
Experimental and Therapeutic Medicine, 14(2), 1809-1817 (2017)
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Experimental and therapeutic medicine, 14(2), 1809-1817 (2017-08-16)
The aim of the present study was to investigate the inhibitory effect of compound cantharides capsules (CCCs) on the viability and apoptosis of human gastric cancer cell lines, BGC-823 and SGC-7901, and to detect its regulation of gene expression levels
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商品
Horseradish peroxidase (HRP) is an enzyme used for detecting molecules, degrading toxins, cross-linking proteins, and in diagnostics like lateral flow assays.
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