Name: MS β-Glucuronidase
Brand: SIGMA

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About This Item

UNSPSC代码:

12352204

NACRES:

NA.54

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质量水平

200

表单

ready-to-use solution

运输

ambient

储存温度

2-8°C

一般描述

Protocol: MS β-Glucuronidase Quick Start Guide
MS β-Glucuronidase is a stable recombinant β-Glucuronidase mix for robust urine drug testing. Hydrolysis is completed at room-temperature or in your autosampler in a single-step . The stability and low-temperature compatibility allows an analyst to use MS β-Gluc as a bench-top reagent. Reliable hydrolysis over days on the bench means less hands-on time, less frequent reagent prep, and more automation. It has been verified against a broad spectrum of conjugated analytes, including “hard to cleave” glucuronides, particularly opiates and opioids. MS β-Gluc eliminates sample preparation bottlenecks while enabling fully automated hydrolysis and reproducible analytical results.

应用

Urine sample preparation in Forensic and Clinical toxicology laboratories
Enzymatic hydrolysis of drug glucuronide conjugates for urine drug testing by LC/MS
Forensic drug testing, workplace and prescription drug monitoring, antidoping, SAMHSA
Compatible with wide range of glucuronide drug conjugates, including but not limited to opioids, opiates, benzodiazepines and cannabinoids and hormones.

特点和优势

Opened bottle retains 97% activity at 25°C after 2 months.
Stable on the bench in MasterMix (with methanol): Retains 99% activity for a week.
Pre-buffered for use with human urine with volatile buffer ammonium acetate for LC-MS.
Single-step hydrolysis at room-temperature or within autosampler (20-40°C).
Verified with real human urine samples, not synthetic.
“Walk-away” workflow.
Automation compatibility (oven and centrifuge not required).
Purified recombinant enzyme

制备说明

For Opiates, Opioids and Broad-spectrum drug hydrolysis use 80 to 160 μL of MS ß-Glucuronidase per reaction for a 200 μL reaction in urine, so 6-12 samples can be hydrolyzed per mL of MS beta-Gluc depending on reaction temperature.

Dilute-and-shoot workflows: add MS β-glucuronidase formula and internal standards in methanol to urine and immediately transfer to autosampler at 40°C. After 16min switch temperature to 4°C. Hydrolyzed samples are ready for injection.

Workflows with extraction or filtration step: add MS β-glucuronidase formula and internal standards in methanol to urine. Incubate 30 min at room-temperature. Proceed with extraction or filtration.