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安全信息

SRE0103

Sigma-Aldrich

MS β-Glucuronidase

Prebuffered, automation-ready hydrolysis mix

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别名:
GUS, MS beta-Glu, beta-Gluc, bgluc

质量水平

200

形式

ready-to-use solution

运输

ambient

储存温度

2-8°C

相关类别

一般描述

  • Protocol: MS β-Glucuronidase Quick Start Guide
  • MS β-Glucuronidase is a stable recombinant β-Glucuronidase mix for robust urine drug testing. Hydrolysis is completed at room-temperature or in your autosampler in a single-step . The stability and low-temperature compatibility allows an analyst to use MS β-Gluc as a bench-top reagent. Reliable hydrolysis over days on the bench means less hands-on time, less frequent reagent prep, and more automation. It has been verified against a broad spectrum of conjugated analytes, including “hard to cleave” glucuronides, particularly opiates and opioids. MS β-Gluc eliminates sample preparation bottlenecks while enabling fully automated hydrolysis and reproducible analytical results.

应用

  • Urine sample preparation in Forensic and Clinical toxicology laboratories
  • Enzymatic hydrolysis of drug glucuronide conjugates for urine drug testing by LC/MS
  • Forensic drug testing, workplace and prescription drug monitoring, antidoping, SAMHSA
  • Compatible with wide range of glucuronide drug conjugates, including but not limited to opioids, opiates, benzodiazepines and cannabinoids and hormones.

特点和优势

  • Opened bottle retains 97% activity at 25°C after 2 months.
  • Stable on the bench in MasterMix (with methanol): Retains 99% activity for a week.
  • Pre-buffered for use with human urine with volatile buffer ammonium acetate for LC-MS.
  • Single-step hydrolysis at room-temperature or within autosampler (20-40°C).
  • Verified with real human urine samples, not synthetic.
  • “Walk-away” workflow.
  • Automation compatibility (oven and centrifuge not required).
  • Purified recombinant enzyme

制备说明

For Opiates, Opioids and Broad-spectrum drug hydrolysis use 80 to 160 μL of MS ß-Glucuronidase per reaction for a 200 μL reaction in urine, so 6-12 samples can be hydrolyzed per mL of MS beta-Gluc depending on reaction temperature.

Dilute-and-shoot workflows: add MS β-glucuronidase formula and internal standards in methanol to urine and immediately transfer to autosampler at 40°C. After 16min switch temperature to 4°C. Hydrolyzed samples are ready for injection.

Workflows with extraction or filtration step: add MS β-glucuronidase formula and internal standards in methanol to urine. Incubate 30 min at room-temperature. Proceed with extraction or filtration.

储存及稳定性

  • Long term Storage: 24 months at 2 - 8 °C.
  • Ambient for 2 months.
  • Ambient for 1 week in Master Mix (11% methanol).

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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商品

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

实验方案

Simple Urine Drug Screening with MS β-glucuronidase: Quick Start Guide and Demo Video

MS β-glucuronidase urinalysis workflow overview, features and benefits, and quick start guide video for your drug testing application needs.

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