General description
Uracil-DNA glycosylases are DNA repair enzymes that participate in the base excision repair pathway. Uracil DNA Glycosylase, also known as UNGor UDG, is an enzyme that hydrolyses the N-glycosylic bond between the deoxyribose sugar and the base in uracil-containing DNA leaving an abasic(apyrimidinic) site in DNA. Contamination of PCR samples with amplicon DNA generated by previous reactions, “carry-over contamination”, is one of the major sources of false positive results. Heat-labile Cod Uracil-DNA Glycosylase prevents carry-over contamination in PCR, qPCR, RT-qPCR, and RT-LAMP assays. The enzyme is produced in a recombinant E. coli (ung–) strain that contains a modified Cod UNGgene.
Application
Heat-labile Cod Uracil-DNA Glycosylase eliminates carry-over contamination in PCR, RT-PCR, RT-qPCR, qPCR and RT-LAMP
Features and Benefits
- Heat inactivation: The only UNG that is completely and irreversibly heat inactivated thus ensuring long-term sample integrity for subsequent post-PCR analysis such as cloning, sequencing or genotyping
- Cod UNG is not active on uracil in RNA.
- High sensitivity: Remove more than 108 copies of contaminating DNA without affecting the sensitivity (Cq) of the assay
- Simple protocol: Applying Cod UNG in a RT-PCR is done by a 5 min Cod UNG preincubation step at RT prior to the amplification reaction
Preparation Note
In solution
Other Notes
One Unit is defined as the amount of enzyme that liberates 1 nmol Uracil per hour from Uracil-labelled DNA at 37 °C in 70 mm Tris-HCI pH 8.0 (@25 °C), 10 mM NaCI, 1 mM EDTA and 0.1 mg/ml BSA.
This product is for R&D use only. Not for drug, household, or other uses.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Uracil-DNA glycosylases?Structural and functional perspectives on an essential family of DNA repair enzymes
N Schormann et al.
Protein Science, 1667?1685-1667?1685 (2014)
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持