SRP0129
Dot1L human
recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
别名:
DOT1-like, histone H3 methyltransferase, H3-K79-HMTase, KIAA1814, KMT4, Lysine N-methyltransferase 4
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352202
biological source
human
recombinant
expressed in E. coli
assay
≥70% (SDS-PAGE)
form
aqueous solution
mol wt
73 kDa
packaging
pkg of 50 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... DOT1L(84444)
General description
Human DOT1L, GenBank Accession No. NM_032482), amino acids 2-416 with N-terminal ST tag, MW = 73 kDa, expressed in E. coli expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 7.5, 100mM NaCl, 0.05% Tween 20, 20% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.