SRP0132
EZH2 / EED / SUZ12 human
recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)
别名:
ENX-1, EZH2, Enhancer of zeste homolog 2, KMT6A, Lysine N-methyltransferase 6A
产品名称
EZH2 / EED / SUZ12 human, recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)
biological source
human
recombinant
expressed in baculovirus infected insect cells
assay
≥90% (SDS-PAGE)
form
aqueous solution
mol wt
51 kDa (EED)
86 kDa (EZH2)
87 kDa (SUZ12)
packaging
pkg of 50 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... EZH2(2146)
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
General description
Complex of: Human EZH2 (GenBank Accession No. NM_004456), (amino acids 2-end) with N-terminal His tag, MW = 86 kDa. Human EED (GenBank Accession No. NM_003797) (amino acid 2-end) with N-terminal Flag tag, MW = 51 kDa. Human SUZ12, NM_015355, amino acids 2-end, with N-terminal His tag.
Other Notes
One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 100 μg/ml flag peptide and 10% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
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