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Merck
CN

SRP0142

Sigma-Aldrich

PRMT3 human

recombinant, expressed in E. coli, ≥60% (SDS-PAGE)

别名:

Arginine methyltransferase 3, HMT1 hnRNP methyltransferase-like 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like 3

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关于此项目

UNSPSC代码:
12352200
NACRES:
NA.32
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生物来源

human

重组

expressed in E. coli

方案

≥60% (SDS-PAGE)

表单

aqueous solution

分子量

86 kDa

包装

pkg of 50 μg

储存条件

avoid repeated freeze/thaw cycles

浓度

>0.02 mg/mL

NCBI登记号

UniProt登记号

运输

dry ice

储存温度

−70°C

基因信息

human ... PRMT3(10196)

一般描述

Human PRMT3, GenBank Accession No. NM_005788, amino acids 2-end, with N-terminal GST tag, MW = 86 kDa, expressed in an E. coli expression system.

应用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

其他说明

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Mamta Verma et al.
Communications biology, 4(1), 109-109 (2021-01-27)
Protein arginine methyltransferase 3 (PRMT3) regulates protein functions by introducing asymmetric dimethylation marks at the arginine residues in proteins. However, very little is known about the interaction partners of PRMT3 and their functional outcomes. Using yeast-two hybrid screening, we identified

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