产品名称
SMYD3 (full length) Active human, recombinant, expressed in baculovirus infected insect cells, ≥50% (SDS-PAGE)
biological source
human
recombinant
expressed in baculovirus infected insect cells
assay
≥50% (SDS-PAGE)
form
aqueous solution
mol wt
50 kDa
packaging
pkg of 20 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... SMYD3(64754)
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
General description
Human SMYD3 (GenBank Accession No. BC031010), full length, with N-terminal DDDDK tag (FLAG), MW = 50 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 100 μg/ml flag peptide, 20% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
法规信息
新产品
此项目有
Ilham M Alshiraihi et al.
International journal of molecular sciences, 21(24) (2020-12-19)
SMYD3 is a lysine methyltransferase that regulates the expression of over 80 genes and is required for the uncontrolled proliferation of most breast, colorectal, and hepatocellular carcinomas. The elimination of SMYD3 restores normal expression patterns of these genes and halts
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