产品名称
Histone H4 peptide (1-21) human, ≥90% (HPLC)
biological source
human
assay
≥90% (HPLC)
form
aqueous solution
mol wt
~2 kDa
packaging
pkg of 160 nmol
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... HIST2H4A(8370)
Application
Study enzyme kinetics, and screen small molecular inhibitors of Histone 3 R3 methyltransferases for drug discovery and HTS applications
Biochem/physiol Actions
H4 (histone 4) is a core histone protein which is mobilized during herpes simplex virus 1 (HSV1) infection. This leads to elevated pools of free H2B and H4 and decreased rate of H2B fast chromatin exchange. Free H4 associates with HSV1 genomes, and this mobilization is also linked with the organization of H2A-H2B and H3-H4 dimers into unstable nucleosomes with HSV-1 genomes. The histone chaperone nuclear autoantigenic sperm protein (NASP) is responsible for maintaining a reservoir of soluble H3-H4 histones, where it regulates the activity of heat shock proteins Hsc70 and Hsp90, which target H3-H4 for degradation by chaperone-mediated autophagy. This reservoir level, in turn, depends upon the level of NASP.
Physical form
Supplied as water solution, 800 μM
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube to recover full content of the tube. Aliquot into single use aliquots. Store remaining product in aliquots at -70°C. Note: Avoid freeze/thaw cycles.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
A specific function for the histone chaperone NASP to fine-tune a reservoir of soluble H3-H4 in the histone supply chain.
Molecular Cell, 44(6), 918-927 (2011)
Core histones H2B and H4 are mobilized during infection with herpes simplex virus 1.
Conn KL, et al.
Journal of Virology, 85(24), 13234-13252 (2011)
The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly.
Ahmad K and Henikoff S
Molecular Cell, 9(6), 1191-1200 (2002)
A comprehensive view of the epigenetic landscape part I: DNA methylation, passive and active DNA demethylation pathways and histone variants.
Sadakierska-Chudy A
Neurotoxicity Research, 27(1), 84-97 (2015)
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