SRP0169
JMJD2B Active human
recombinant, expressed in baculovirus infected insect cells, ≥40% (SDS-PAGE)
别名:
JHDM3B, JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, KIAA0876, Lysine-specific demethylase 4B (KDM4B)
产品名称
JMJD2B Active human, recombinant, expressed in baculovirus infected insect cells, ≥40% (SDS-PAGE)
biological source
human
recombinant
expressed in baculovirus infected insect cells
assay
≥40% (SDS-PAGE)
form
aqueous solution
mol wt
57 kDa
packaging
pkg of 100 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... KDM4B(23030)
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
General description
Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No.NM_015015) (amino acids 1-500) with C-terminal FLAG tag, MW = 57 kDa, expressed in Sf9 cells using a Baculovirus expression system.
Other Notes
One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37°C.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
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