产品名称
组蛋白H3全长 人, recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
biological source
human
recombinant
expressed in E. coli
assay
≥80% (SDS-PAGE)
form
aqueous solution
mol wt
15.4 kDa
packaging
pkg of 1 mg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
technique(s)
activity assay: suitable
solubility
water: soluble
suitability
suitable for molecular biology
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... HIST1H3E(8353)
Physical form
溶于10 mM PBS，pH 7.4，含130 mM NaCl、10%甘油和1mM DTT。
Application
人类全长组蛋白H3已作为底物用于Aurora-A（AURKA）激酶和Tousled样激酶2（TLK2）的体外激酶测定。人类全长组蛋白H3已用于鸡抗体的产生。它还用于体外甲基化测定。
Biochem/physiol Actions
组蛋白对于染色质的正确组织和修饰很重要。 HIST1H3E（组蛋白3，也称为H3.1）主要与DNA复制过程中的DNA合成有关，也可能与DNA修复有关。 在与DNA复制相关的染色质组装时，它被添加到染色质中。在HSV-1（单纯疱疹病毒1型）感染期间，HIST1H3E的差异运动与病毒染色质的组装有关。
General description
研究领域：细胞信号传导。基因HIST1H3E（组蛋白 3，也称为H3.1）定位于人类染色体6p22。它是组蛋白3的变体，也称为复制组蛋白。HIST1H3E主要在S期表达。在细胞分化过程中，HIST1H3E的mRNA水平随着细胞分裂的减慢而下降。人HIST1H3E，GenBank登录号NM_003532，氨基酸2-137（末端）带有N端His标签，MW = 15.4 kDa，在大肠杆菌表达系统中表达。
Preparation Note
在冰上解冻。第一次解冻后，短暂旋转含有蛋白质的试管以溶解管内全部内容物。将蛋白质分装成一次性使用的分量。将剩余的未稀释蛋白质分装并保存在-70°C下。注意：蛋白质对冻融循环非常敏感。
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Histone H3.1 and H3.3 complexes mediate nucleosome assembly pathways dependent or independent of DNA synthesis.
Tagami H
Cell, 116, 51-61 (2004)
Method of Immunoglobulin Y (IgY) Extraction for Minimally Toxic In Vivo Applications
Nevado Jr, et al.
Philippine journal of Science, 153(1) (2024)
The human histone H3 complement anno 2011.
Ederveen TH
Biochimica et Biophysica Acta, 1809, 577-586 (2011)
Jona Merx et al.
Scientific reports, 12(1), 19251-19251 (2022-11-11)
Posttranslational modifications (PTMs) on histone tails regulate eukaryotic gene expression by impacting the chromatin structure and by modulating interactions with other cellular proteins. One such PTM has been identified as serine and threonine glycosylation, the introduction of the ß-N-acetylglucosamine (GlcNAc)
Kami Ahmad et al.
Molecular cell, 9(6), 1191-1200 (2002-06-28)
Two very similar H3 histones-differing at only four amino acid positions-are produced in Drosophila cells. Here we describe a mechanism of chromatin regulation whereby the variant H3.3 is deposited at particular loci, including active rDNA arrays. While the major H3
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