biological source
human
recombinant
expressed in E. coli
assay
≥80% (SDS-PAGE)
form
aqueous solution
mol wt
12.1 kDa
packaging
pkg of 1 mg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... HIST2H4A(8370)
General description
Human Histone 4 (HIST2H4A) (GenBank Accession No. NM_003548), amino acids 2-104 (end) with N-terminal His-tag, MW = 12.1 kDa, expressed in an E. coli expression system.
Application
Suitable substrate for histone methyltransferases.
Biochem/physiol Actions
Histones H2B and H4 are found to become mobilized during an infection with HSV-1 (herpes simplex virus-1) and become available for binding to viral genome.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing protein to recover full content of the tube. Aliquot protein into single use aliquots. Store remaining undiluted protein in aliquots at -70°C. Note: Protein is very sensitive to freeze/thaw cycles.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable