SRP0198
PARP11 Active human
recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)
别名:
C12orf6, DKFZp779H0122, MIB006, Poly (ADP-ribose) Polymerase 11
产品名称
PARP11 Active human, recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)
biological source
human
recombinant
expressed in baculovirus infected insect cells
assay
≥80% (SDS-PAGE)
form
aqueous solution
mol wt
65.6 kDa
packaging
pkg of 10 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PARP11(57097)
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
General description
Human PARP11 (GenBank Accession No. NM_020367), full length with N-terminal GST tag, MW = 65.6 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Other Notes
One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into acid-insoluble form.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
存储类别
10 - Combustible liquids
wgk
WGK 1
法规信息
常规特殊物品
此项目有
Mirella L Meyer-Ficca et al.
Biology of reproduction, 92(3), 80-80 (2015-02-13)
Sperm are highly differentiated cells characterized by their species-specific nuclear shapes and extremely condensed chromatin. Abnormal head shapes represent a form of teratozoospermia that can impair fertilization capacity. This study shows that poly(ADP-ribose) polymerase-11 (ARTD11/PARP11), a member of the ADP-ribosyltransferase
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