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NACRES:
NA.32
UNSPSC Code:
12352200
Form:
aqueous solution
Assay:
≥60% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in baculovirus infected insect cells
Mol wt:
74 kDa
产品名称
cMet Active human, recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)
biological source
human
recombinant
expressed in baculovirus infected insect cells
assay
≥60% (SDS-PAGE)
form
aqueous solution
mol wt
74 kDa
packaging
pkg of 10 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... MET(4233)
Application
Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.
General description
Human cMet (GenBank Accession No. X54559), (amino acids 956-1390) with N-terminal GST tag, MW = 74 kDa, expressed in an Baculovirus infected Sf9 cell expression system.
Other Notes
One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 130 mM NaCl, 0.05% Tween-20, 3 mM DTT and 20% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
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