SRP0243
aRAF Active human
recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)
别名:
A-Raf proto-oncogene, PKS2, v-Raf murine sarcoma 3611 viral oncogene homolog
一般描述
Human A-RAF (GenBank Accession No. NM_001654), amino acids 282-end, containing the mutations YY301-302DD, MW = 63.6 kDa, with N-terminal GST tag, expressed in an Baculovirus infected Sf9 cell expression system.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
法规信息
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