SRP0243
aRAF Active human
recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)
别名:
A-Raf proto-oncogene, PKS2, v-Raf murine sarcoma 3611 viral oncogene homolog
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352202
biological source
human
recombinant
expressed in baculovirus infected insect cells
assay
≥80% (SDS-PAGE)
form
aqueous solution
mol wt
63.6 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... ARAF(369)
General description
Human A-RAF (GenBank Accession No. NM_001654), amino acids 282-end, containing the mutations YY301-302DD, MW = 63.6 kDa, with N-terminal GST tag, expressed in an Baculovirus infected Sf9 cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.