产品名称
STK33 Active human, recombinant, expressed in baculovirus infected insect cells, ≥65% (SDS-PAGE)
biological source
human
recombinant
expressed in baculovirus infected insect cells
assay
≥65% (SDS-PAGE)
form
aqueous solution
mol wt
84 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... STK33(65975)
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
General description
STK33 (GenBank Accession No. NM_030906.2), amino acids 2-end, with N-terminal GST-tag, MW = 84 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.
Other Notes
One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of MBP substrate per minute at room temperature
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
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