SRP0257
PDE1A Active human
recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)
别名:
Calcium/calmodulin-dependent 3′,5′-cyclic nucleotide phosphodiesterase 1A, HDAM1, HSPDE1A, Phosphodiesterase 1A
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352200
Specific activity:
≥1237 units/μg
Assay:
≥70% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in baculovirus infected insect cells
Concentration:
>0.02 mg/mL
biological source
human
recombinant
expressed in baculovirus infected insect cells
assay
≥70% (SDS-PAGE)
form
aqueous solution
specific activity
≥1237 units/μg
mol wt
88.3 kDa
packaging
pkg of 10 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PDE1A(5136)
General description
Human PDE1A (GenBank Accession No. NM_005019), full length with N-terminal GST tag, MW = 88.3 kDa, expressed in a Baculovirus infected Sf9 cell expression system
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C