一般描述
Human PDE1B (GenBank Accession No. NM_000924), full length with N-terminal GST tag, MW = 87 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
单位定义
One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
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