SRP0276
PDE10A Active from mouse
recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)
别名:
Dual-substrate cAMP and cGMP, cyclic nucleotide phosphodiesterase, PDE10A2 brain-specific isoform, Phosphodiesterase 10A
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352200
Assay:
≥80% (SDS-PAGE)
Biological source:
mouse
Recombinant:
expressed in baculovirus infected insect cells
Concentration:
>0.02 mg/mL
biological source
mouse
recombinant
expressed in baculovirus infected insect cells
assay
≥80% (SDS-PAGE)
form
aqueous solution
mol wt
114.5 kDa
packaging
pkg of 10 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
mouse ... PDE10A(23984)
General description
Mouse PDE10A GenBank Accession No. NM_011866), full length with N-terminal GST tag, MW = 114.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C